Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy
2011 (English)In: BMC neuroscience (Online), ISSN 1471-2202, Vol. 12, 16- p.Article in journal (Refereed) Published
Background: The Na+,K+-ATPase plays an important role for ion homeostasis in virtually all mammalian cells, including neurons. Despite this, there is as yet little known about the isoform specific distribution in neurons. Results: With help of superresolving stimulated emission depletion microscopy the spatial distribution of Na+,K+-ATPase in dendritic spines of cultured striatum neurons have been dissected. The found compartmentalized distribution provides a strong evidence for the confinement of neuronal Na+,K+-ATPase (alpha 3 isoform) in the postsynaptic region of the spine. Conclusions: A compartmentalized distribution may have implications for the generation of local sodium gradients within the spine and for the structural and functional interaction between the sodium pump and other synaptic proteins. Superresolution microscopy has thus opened up a new perspective to elucidate the nature of the physiological function, regulation and signaling role of Na+,K+-ATPase from its topological distribution in dendritic spines.
Place, publisher, year, edition, pages
2011. Vol. 12, 16- p.
IdentifiersURN: urn:nbn:se:kth:diva-31019DOI: 10.1186/1471-2202-12-16ISI: 000287447600001PubMedID: 21272290ScopusID: 2-s2.0-79251589617OAI: oai:DiVA.org:kth-31019DiVA: diva2:402231
FunderSwedish Research Council, VR-2006-3197, VR-2007-4582, VR-2007-2519EU, FP7, Seventh Framework Programme, Fluodiamon 201 837Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201103072011-03-072011-03-072014-02-07Bibliographically approved