Extraction of therapeutic proteins from dried blood spots and their analysis on Gyrolab
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
A method for extraction of therapeutic proteins from dried blood spots (DBS) followed by quantification on Gyrolab(TM) has been developed. The method makes it possible to measure the concentration of the analyte in the range 100-6000 ng/mL. The procedure can generate full analytical information from 15 μL blood originally sampled from a subject. The modest sample requirements allows for sampling a full pre-clinical pharmacokinetic profile from a single mouse. This may allow for reduced usage of animals during preclinical development of new therapeutic proteins in accordance with the 3R’s, replace, refine and reduce.
Place, publisher, year, edition, pages
2011. , 49 p.
UPTEC X, 11 004
Dried blood spots, immunoassays, therapeutic proteins, monoclonal antibodies, Gyrolab
Other Industrial Biotechnology Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:uu:diva-144920OAI: oai:DiVA.org:uu-144920DiVA: diva2:394790