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Extraction of therapeutic proteins from dried blood spots and their analysis on Gyrolab
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2011 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

A method for extraction of therapeutic proteins from dried blood spots (DBS) followed by quantification on Gyrolab(TM) has been developed. The method makes it possible to measure the concentration of the analyte in the range 100-6000 ng/mL. The procedure can generate full analytical information from 15 μL blood originally sampled from a subject. The modest sample requirements allows for sampling a full pre-clinical pharmacokinetic profile from a single mouse. This may allow for reduced usage of animals during preclinical development of new therapeutic proteins in accordance with the 3R’s, replace, refine and reduce.

Place, publisher, year, edition, pages
2011. , 49 p.
Series
UPTEC X, 11 004
Keyword [en]
Dried blood spots, immunoassays, therapeutic proteins, monoclonal antibodies, Gyrolab
National Category
Other Industrial Biotechnology Other Industrial Biotechnology
Identifiers
URN: urn:nbn:se:uu:diva-144920OAI: oai:DiVA.org:uu-144920DiVA: diva2:394790
Uppsok
Technology
Supervisors
Available from: 2011-03-09 Created: 2011-02-03 Last updated: 2011-03-09Bibliographically approved

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Biology Education Centre
Other Industrial BiotechnologyOther Industrial Biotechnology

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ReferencesLink to record
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