Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Imaging phosphoinositide dynamics in living cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2010 (English)In: Inositol Phosphates and Lipids: Methods and Protocols / [ed] Christopher J. Barker, Humana Press , 2010, Vol. 645, p. 219-235Chapter in book (Other academic)
Abstract [en]

To improve our understanding of the important roles played by inositol lipid derivatives in signalling and other cellular processes, it is crucial to measure phosphoinositide concentration changes in individual cells with high spatial and temporal resolution. A number of protein domains that interact with inositol lipids in a specific manner have been identified. Tagged with the green fluorescent protein or its colour variants, these protein modules can be used as probes to visualize various phosphoinositide species in different sub-cellular compartments. Here, we present protocols for fluorescence imaging of phosphoinositide dynamics in single living cells. Total internal reflection fluorescence microscopy is particularly powerful for time-lapse recordings of phosphoinositides in the plasma membrane. We demonstrate how this technique can be used to record phospholipase C- and PI3-kinase-induced changes in inositol lipids in insulin-secreting cells. These procedures should be applicable to studies of the spatio-temporal regulation of phosphoinositide metabolism in many types of cells.

Place, publisher, year, edition, pages
Humana Press , 2010. Vol. 645, p. 219-235
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 645
Keyword [en]
phosphatidylinositol 4, 5-bisphosphate, phosphatidylinositol 3, 4, 5-trisphosphate, phospholipase C, PI3-kinase, pleckstrin homology domain, Ca2+, green fluorescent protein, total internal reflection fluorescence microscopy, insulin-secreting cell
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-141217DOI: 10.1007/978-1-60327-175-2_14ISBN: 978-1-60327-174-5 (print)ISBN: 978-1-60327-175-2 (print)OAI: oai:DiVA.org:uu-141217DiVA, id: diva2:385277
Funder
Swedish Research Council
Available from: 2011-01-12 Created: 2011-01-11 Last updated: 2011-11-23Bibliographically approved

Open Access in DiVA

fulltext(504 kB)533 downloads
File information
File name FULLTEXT02.pdfFile size 504 kBChecksum SHA-512
28aba4a1487786d9b35ad828bee9c580f8b8ff7c3796d6483d17df6ebc8edd2d2269ee63f311d79de3314bec25452cc5b2a0b2cfd402864f50ee324f34b12005
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Wuttke, AnneTengholm, Anders
By organisation
Department of Medical Cell Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 533 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
isbn
urn-nbn

Altmetric score

doi
isbn
urn-nbn
Total: 744 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
v. 2.34-SNAPSHOT
|