Change search
ReferencesLink to record
Permanent link

Direct link
Validation of suitable endogenous control genes for expression studies of miRNA in prostate cancer tissues
Örebro University, School of Health and Medical Sciences.
Show others and affiliations
2010 (English)In: Cancer Genetics and Cytogenetics, ISSN 0165-4608, E-ISSN 1873-4456, Vol. 202, no 2, 71-75 p.Article in journal (Refereed) Published
Abstract [en]

When performing quantitative polymerase chain reaction analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used: small nuclear RNAs and small nucleolar RNAs. In this study, six different endogenous control genes for miRNA studies were investigated in prostate tissue material from the Swedish Watchful Waiting cohort. The stability of the controls was investigated using two different software applications, NormFinder and BestKeeper. RNU24 was the most suitable endogenous control gene for miRNA studies in prostate tissue materials. (C) 2010 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2010. Vol. 202, no 2, 71-75 p.
National Category
Medical and Health Sciences
Research subject
URN: urn:nbn:se:oru:diva-12861DOI: 10.1016/j.cancergencyto.2010.06.009ISI: 000282862400001OAI: diva2:384504
Available from: 2012-09-17 Created: 2011-01-03 Last updated: 2012-11-01Bibliographically approved
In thesis
1. Identification of miRNA expression profiles for diagnosis and prognosis of prostate cancer
Open this publication in new window or tab >>Identification of miRNA expression profiles for diagnosis and prognosis of prostate cancer
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Cancer of the prostate (CaP) is the most common malignancy diagnosed in men in the Western society. During the last years, prostate specific antigen (PSA) has been used as a biomarker for CaP, although a high PSA value is not specific for CaP. Thus, there is an urgent need for new and improved diagnostic markers for CaP.

In this thesis, the aim was to find a miRNA signature for diagnosis of CaP and to elucidate if differences in behavior between transition zone and peripheral zone tumors are reflected in miRNA expression. One of the major findings is anexpression signature based on nine miRNAs that with high accuracy (85%) could classify normal and malignant tissues from the transition zone of the prostate. The results furthermore show that the major differences in miRNA expression are found between normal and malignant tissues, rather than between the different zones. In addition, tumors arising in the peripheral zone have fewer changes in miRNA expression compared to tumors in the transition zone, indicating that the peripheral zone is more prone to tumor development compared to the transition zone of the prostate.

A crucial step in pre-processing of expression data, in order to differentiate true biological changes, is the normalization step. Therefore, an additional aim of this thesis was to compare different normalization methods for qPCR array data in miRNA expression experiments. The results show that data-driven methods based on quantile normalization performs the best. The results also show that in smaller miRNA expression studies, only investigating a few miRNAs, RNU24 is the most suitable endogenous control gene for normalization.

Taken together, the results in this thesis show the importance of miRNAs and the possibility of their future use as biomarkers in the field of prostate cancer.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2012. 55 p.
Örebro Studies in Medicine, ISSN 1652-4063 ; 74
Prostate cancer, microRNAs, prostate zones, normalization, endogenous controls
National Category
Medical and Health Sciences Cancer and Oncology
Research subject
urn:nbn:se:oru:diva-25600 (URN)978-91-7668-888-5 (ISBN)
Public defence
2012-10-26, Wilandersalen, Universitetssjukuset (USÖ), Örebro, 13:00 (Swedish)
Available from: 2012-08-30 Created: 2012-08-30 Last updated: 2016-04-19Bibliographically approved

Open Access in DiVA

Manuscript before referee(72 kB)189 downloads
File information
File name FULLTEXT01.pdfFile size 72 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Carlsson, Jessica
By organisation
School of Health and Medical Sciences
In the same journal
Cancer Genetics and Cytogenetics
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 189 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 43 hits
ReferencesLink to record
Permanent link

Direct link