Immunological Effects of TBE Vaccination: Increased Expression of Transcription factor T-bet Indicates Activation of Th1-like Cellular Immunity
Independent thesis Advanced level (professional degree), 15 credits / 22,5 HE creditsStudent thesis
Tick-borne encephalitis virus is the cause of much morbidity and sometimes a fatal infection. A vaccine based on formaldehyde inactivated virus is currently the only available way of preventing disease. This vaccine gives a high rate of seroconversion but there are reports of vaccination breakthrough, even in people who have demonstrated a neutralizing antibody response. The T cell response to inactivated TBE vaccine is largely unknown, but could be of importance for the effect of the vaccine. This study characterizes aspects of the T cell response by investigating the expression of two transcription factors, T-bet and GATA-3 with RT-PCR. T-bet is expressed in CD4+ T cells of the Th1 type, while GATA-3 is expressed in CD4+ T cells of the Th2 type. Our data show that vaccination with inactivated TBE vaccine leads to increase in expression of the T-bet gene when cells of vaccinated subjects are cultured with TBE virus. In contrast, the expression of GATA-3 remains unaffected by vaccination. Thus, this study suggests that the inactivated TBE vaccine leads to a Th1-like immune response in humans.
Place, publisher, year, edition, pages
2007. , 24 p.
TBE, Vaccination, T-cell, T-bet, GATA-3, Th1, Th2
Immunology in the medical area
IdentifiersURN: urn:nbn:se:liu:diva-59537ISRN: -OAI: oai:DiVA.org:liu-59537DiVA: diva2:352209
Ernerudh, Jan, Professor
Påhlsson, Peter, Professor