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Analysis of tumour infiltrating leukocytes in colon cancer carcinoma in a syngeneic rat model
Linköping University, Department of Physics, Chemistry and Biology. (RIT-gruppen)
2010 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Tumour immunity is a balance between immune mediators that promote tumor progression versus mediators that promote tumor rejection. Infiltrating lymphocytes in human colorectal cancer tissues are independent prognostic factors for a better survival and a high number of cytotoxic CD8+ T-cells have been associated with a better prognosis in terms of a longer and disease free survival for the patient. In our syngeneic rat model we induce colon carcinoma subperitoneally by injecting a colon cancer cell line BN7005, a cell line expressing the epitope (Lewis Y) for the BR96 antibody. Tumours are dissected out and treated with different fixatives and then either frozen, snap-frozen or embedded in paraffin followed by sectioning. Immunohistochemistry using monoclonal antibodies against the tumour infiltrating leukocytes was performed on the tissue.

The results were seen as an infiltration of different leukocytes in the tumours.

 

Place, publisher, year, edition, pages
2010. , 31 p.
Keyword [en]
Tumour infiltrating leukocytes, Immunohistochemistry, Colon cancer, monoclonal antibodies, BR96 antibody
National Category
Cancer and Oncology Medical Genetics Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-56910ISRN: LITH-IFM-A-Ex--10/2230--SEOAI: oai:DiVA.org:liu-56910DiVA: diva2:323007
Uppsok
Medicine
Supervisors
Available from: 2011-02-28 Created: 2010-06-09 Last updated: 2011-02-28Bibliographically approved

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Borgström, Annelie
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