Movement behaviors research has evolved from focusing on single behaviors to multiple behaviors within a 24-h perspective. However, it is unknown if 24-h movement behavior profiles are consistently associated across multiple health outcomes. Thus, we aimed to investigate this. We used data from 807 adults who wore thigh accelerometers and recorded daily sleep/work times over 1–4 days and were categorized into four 24-h movement behavior profiles: “Chimpanzees” (balanced distribution of movement behaviors in work and leisure; n = 226, reference), “Lions” (more active work and sleep, and less active leisure; n = 179), “Ants” (more active overall, less sedentary work and similar sleep, n = 244), and “Koalas” (more sedentary and sleep, and less active overall n = 158). Cardiorespiratory fitness and systolic blood pressure were measured, while low back pain and self-rated health were self-reported. Linear or ordinal logistic regression assessed the cross-sectional associations between these profiles and outcomes, adjusting for age, sex, BMI, smoking, alcohol, occupational lifting/carrying, and work type. We found that referencing Chimpanzees, Lions were detrimentally associated with cardiorespiratory fitness (B = −2.70 mLO2/min/kg, p < 0.01), but beneficially associated with systolic blood pressure (B = −3.49 mmHg, p < 0.05) and low back pain (odds ratio, OR = 0.67, p = 0.03). Koalas were detrimentally associated with systolic blood pressure (B = 3.66 mmHg, p < 0.05) and cardiorespiratory fitness (B = −2.83 mLO2/min/kg, p < 0.01). Ants were detrimentally associated with self-reported health (OR = 1.78, p < 0.01). We conclude that no 24-h movement behavior profile was consistently (i.e., solely beneficial or detrimental) associated with the health outcomes. These findings indicate that research and practice about 24-h movement behaviors need to consider multiple outcomes.