This study is positioned in a multidisciplinary research field addressing questions of innovation, foresight, risk perception, regulation, and the role of stakeholder experts as regards nanomaterials and nanotechnology. Nanotechnology is an innovative scientific field with many potential societal benefits but also high uncertainty about risks to human health and the environment. This study is based on a survey distributed to a sample of 237 expert stakeholders in Sweden working in the field of nanotechnology innovation and regulation. The sample comprises experts in both industry and government organizations. The paper explores the expert’s assessment of benefits, risks, and their views of nanotechnology regulation. The experts generally agreed on the need for further regulation of nanotechnology, although they differed in their support for different regulatory measures. Support for government regulation was increased by greater perceived risk and by ethical concerns, while perceived benefit decreased support for government regulation. If nanotechnology was important for the respondent’s organization of affiliation, support for government regulation decreased. Experts in government organizations were more in favor of stronger government regulation, perceived higher risks, and were more concerned about the ethical implications of nanotechnology than were the industry experts. While previous research has discussed views of experts, as well as comparing the attitudes of the general public with experts, this study contributes to the field by analyzing and identifying differences between industry experts and experts working in government.