Simply by existing, we are continuously exposed to potentially harmful chemicals present in our environment. The presence of endocrine-disrupting chemicals poses a risk, as they have the potential to interfere with systems that regulate human development and function, even at low concentrations. Triphenyl phosphate (TPHP) is a suggested developmental neurotoxicant, prevalent in house dust and plastics. Its presence in human milk and placenta raises concern about potential risks to the developing fetus. In a step to evaluate TPHP’s potential for endocrine induced developmental neurotoxicity, C17.2 cells were exposed to concentrations between 50 μM - 0.005 nM in order to cover human exposure levels. The exposure occurred for 10 days of differentiation and the cell viability, neuronal morphology (neurite outgrowth and branching) and neuronal differentiation was assessed. The treatment did not significantly affect cell viability or neuronal differentiation and morphology. However, an insignificant increase in neurite outgrowth and branching was observed, suggested to be a sign for retinoic acid receptor antagonism. Our findings indicate that TPHP is not manifesting developmental neurotoxicity by affecting the tested endpoints under the described conditions. To uncover the mechanism a broader range of endpoints should be assessed.