The ecological connectivity across Europe is crucial for the integrity of ecosystems and the maintaining of biodiversity. Despite this, human development and fragmentation continue to threaten the connectivity within and between the Natura 2000 network of protected areas. I investigated the ecological connectivity within the Doñana area in Southern Spain, focusing on this section of the Natura 2000 network and its surrounding landscape as part of the EU project NaturaConnect. I first assess current connectivity levels in the Doñana area using structural connectivity metrics as well as resistance raster and cumulative current maps. I use these analyses to explore the impact of human activity and infrastructure on ecological connectivity, highlighting areas of high and low connectivity. I also examine the viability of habitat banking as a land management strategy to enhance connectivity through a qualitative literature review. The results show notable variability in connectivity across different land cover types and areas within the study site. The results also suggest that the surrounding landscape contributes to ecological connectivity within the Natura 2000 network. Specific regions, particularly urban and coastal areas, show decreased connectivity due to human impact. The Natura 2000 network does not show elevated rates of connectivity compared to the surrounding landscape. Hot spots of high connectivity/flow are revealed by the cumulative current mapping. From the literature review, I conclude that habitat banking, combined with existing management plans, could potentially improve ecological connectivity in the Doñana area, although implementation challenges remain. These mainly pertain to a lack of political will and the high requirements of the necessary structures for reliably controlling and enforcing offsetting measures. These findings contribute to a broader understanding of landscape connectivity within Doñana to provide insights for conservation. This, along with the findings on the viability of habitat banking will help guide future efforts of NaturaConnect and beyond.