Thermophilic biological syngas methanation has the potential to become an important factor to reduce the usage of fossil fuels and contribute to a resilient energy production in Europe. The technology is built on the complex syntropy of different groups of microbes that together convert syngas (CO, H2, CO2, and some CH4) to CH4 through a variety of pathways. The pathway for CO conversion to CH4 has been less studied than the conversion for H2 and CO2, but several studies have observed that species that perform hydrogenotrophic methanogenesis and the water-gas shift reaction (WGSR) seem to be dominating in biological syngas methanation in thermophilic temperatures. In future commercial plants for biological syngas methanation, the process will perhaps be disturbed by varying amounts of pyrolysis water, a condensate that might enter the reactor with the syngas. Research Institutes of Sweden (RISE) has conducted an experiment where a trickle bed reactor (TBR) has been exposed to pyrolysis water without seeing any apparent effect on the CH4 production. However, after conducting a qPCR analysis, targeting the genera Methanobacterium, on samples from the TBR, it is possible to conclude that the introduction of the contamination indeed had an effect on the methanogenic community since the population decreased at the top of the reactor, where the contamination was decreased. The reason why this was not apparent on the data gathered from the experiment might be because an inoculum which had been thoughtfully chosen with the diversity in mind had been used, and there were plenty of species that could convert the harmful components. This might have protected the lower parts of the reactor while other methanogenic species than Methanobacterium maintained the CH4 production.