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Initiation of Innate Immune Responses in the Freshwater Crayfish Pacifastacus leniusculus
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Evolutionary Biology.
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Prophenoloxidase (proPO) is a key enzyme for generation of melanin and is activated by the proPO activating enzyme (ppA) to its active form, PO. The active ppA was purified and cloned from crayfish hemocytes and it is a typical serine proteinase containing a clip, a proline-rich, and a glycine-rich domain. A recombinant protein containing the clip-domain, with homology to horseshoe crab big defensin and mammalian â-defensin, had antibacterial activity in vitro against gram-positive bacteria.

The proPO activating system (proPO system) is triggered by lipopolysaccharides (LPS) or â-1,3-glucans. An LPS and â-1,3-glucan binding protein (LGBP) was characterized from crayfish hemocytes. The results of an LGBP antibody inhibition assay suggest that LGBP is directly involved in the proPO system.

The primary structure of a crayfish masquerade-like (mas) protein has homology to serine proteinases except for a substitution within the catalytic triad, which renders it without proteinase activity. The crayfish mas-like protein has also binding activity to various gram-negative bacteria and yeast. When the mas-like protein binds to microorganisms, it is processed by a proteolytic enzyme. The mas-like protein exhibited cell adhesion and opsonic activities suggesting that it plays a role in defense against parasites.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 2001. , p. 42
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 613
Keyword [en]
Developmental biology, pattern recognition protein, innate immunity, crayfish
Keyword [sv]
Utvecklingsbiologi
National Category
Developmental Biology
Research subject
Physiological Mycology
Identifiers
URN: urn:nbn:se:uu:diva-623ISBN: 91-554-4973-5 (print)OAI: oai:DiVA.org:uu-623DiVA, id: diva2:167477
Public defence
2001-04-11, Department of Comparative Physiology, Evolutinary Biology Centre, Uppsala University, Norbyvagen 18A, SE-75236, Uppsala, Sweden, Uppsala, 13:00
Available from: 2001-03-21 Created: 2001-03-21Bibliographically approved

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