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White Spot Syndrome Virus Interaction with a Freshwater Crayfish
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Viruses are very abundant in water and hence diseases caused by viruses are common in marine organisms. These diseases create great problems for the commercial farming of crustaceans and mussels. One of the most common and most disastrous diseases for shrimp is caused by the white spot syndrome virus (WSSV), which is spread all around the world and also is infecting many different species of crustaceans including freshwater crayfish. Although during recent years knowledge has been gathered on the ways in which invertebrates defend themselves against bacteria and fungi virtually nothing is known about the defence processes elicited by virus. The aim of this work was to develop a model to use for studies of virus-host interactions in vivo and in vitro.

Temperature was found to be important for the virus infectivity and at lower temperature the virus apparently did not replicate, but if animals kept at low temperature for more than 40 days were transferred to higher temperatures they died quickly due to an increased virus replication. In crayfish infected with the virus it was found that hemocytes did not degranulate and the melanization reaction was also inhibited in the hemocyes. Thus it is apparent that this virus interacts with the immune system and hemocytes in particular and to be able to study this in some greater detail it was necessary to develop a cell culture to study virus-host interactions at the molecular level. Hence, we have developed a stem cell culture from the hematopoietic tissue (hpt) that will differentiate and mature into hemocytes and which can be used to replicate the WSSV in the presence of an endogenous cytokine, astakine. Astakine is the first cytokine like-factor described which is directly involved in hematopoiesis in an invertebrate.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 2005. , p. 56
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 47
Keywords [en]
Biology, WSSV, crayfish, hematopoietic tissue, cytokine, innate immunity, hemocytes
Keywords [sv]
Biologi
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-5776ISBN: 91-554-6235-9 (print)OAI: oai:DiVA.org:uu-5776DiVA, id: diva2:166336
Public defence
2005-05-10, Lindahlsalen, Evolution Biology Centre, Norbyvagen 18A, Uppsala, 10:00 (English)
Opponent
Supervisors
Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2009-04-03Bibliographically approved
List of papers
1. Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus Leniusculus
Open this publication in new window or tab >>Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus Leniusculus
2001 (English)In: Diseases of Aquatic Organisms, ISSN 0177-5103, Vol. 47, p. 151-157Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-92990 (URN)
Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2009-04-05Bibliographically approved
2. WSSV interaction with crayfish hemocytes
Open this publication in new window or tab >>WSSV interaction with crayfish hemocytes
(English)Manuscript (Other (popular science, discussion, etc.))
Identifiers
urn:nbn:se:uu:diva-92991 (URN)
Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2010-01-14Bibliographically approved
3. Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish
Open this publication in new window or tab >>Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish
2004 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, Vol. 17, p. 265-275Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-92992 (URN)
Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2009-04-05Bibliographically approved
4. An ancient role for a prokineticin domain in invertebrate hematopoiesis
Open this publication in new window or tab >>An ancient role for a prokineticin domain in invertebrate hematopoiesis
Show others...
2005 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 174, no 10, p. 6153-6160Article in journal (Refereed) Published
Abstract [en]

Hemopoietic development requires firm control of cell proliferation and differentiation. Although recent research has revealed conserved function of transcription factors and signaling pathways regulating lineage commitment in hemopoietic development in Drosophila melanogaster and vertebrates, little is known about hemopoietic cytokines among the invertebrate phyla. In the present study, we show that differentiation and growth of hemopoietic stem cells in vitro from an invertebrate, Pacifastacus leniusculus, require an endogenous cytokine-like factor, astakine, containing a prokineticin (PK) domain. Astakine induces a strong hematopoiesis response in live animals. An astakine homologue was also found in the shrimp, Penaeus monodon. So far, PK domains are only identified in vertebrates, in which they, for example, direct angiogenic growth. Our finding of the first PK-like cytokine characterized from any invertebrate provides novel information concerning the evolution of growth factors and blood cell development.

Keywords
Amino Acid Sequence, Animals, Astacoidea, Base Sequence, Cell Differentiation/physiology, Cell Division/physiology, Cell Movement/physiology, Cells; Cultured, Cloning; Molecular, Cytokines/genetics/*isolation & purification/*physiology/secretion, Cytoplasmic Granules/secretion, Evolution; Molecular, Hematopoiesis/genetics/*physiology, Hematopoietic Stem Cells/cytology/physiology, Hemocytes/cytology/physiology/secretion, Molecular Sequence Data, Penaeidae, Protein Structure; Tertiary/genetics, Research Support; Non-U.S. Gov't, Sequence Homology; Amino Acid, Structural Homology; Protein, Vascular Endothelial Growth Factor; Endocrine-Gland-Derived/chemistry/genetics/isolation & purification/*physiology/secretion
National Category
Natural Sciences
Identifiers
urn:nbn:se:uu:diva-92993 (URN)15879111 (PubMedID)
Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2017-12-14
5. Replication of white spot syndrome virus (WSSV) in in vitro cultured hematopoietic cell of freshwater crayfish Pacifastacus leniusculus
Open this publication in new window or tab >>Replication of white spot syndrome virus (WSSV) in in vitro cultured hematopoietic cell of freshwater crayfish Pacifastacus leniusculus
(English)Manuscript (Other (popular science, discussion, etc.))
Identifiers
urn:nbn:se:uu:diva-92994 (URN)
Available from: 2005-04-14 Created: 2005-04-14 Last updated: 2010-01-14Bibliographically approved

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