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Expressions of mercury-selenium interaction in vitro
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Interaction between mercury and selenium has previously been observed both in man and in animals. The aim of this work was to study expressions of interaction between mercury and selenium in human K-562 cells. Inorganic and organic forms of mercury and selenium were used and cells were either pre-treated with selenium or simultaneously exposed to selenium and mercury. Concentrations of selenium and mercury chosen were indicated by a study of growth inhibition in the individual compounds: a low concentration of selenium and selenomethionine induced slight cell growth inhibition, while a high concentration resulted in a notable growth inhibition. Two mercury concentrations were chosen: one with minimal toxicity and another with high cell toxicity. In addition, uptake and retention patterns of selenomethionine and selenite differed in both selenocompounds.

All simultaneous treatments with 3.5 μM methylmercury produced a reduction in cellular mercury with increased selenium concentration. This was particularly obvious in selenite treatments. Growth curves from the simultaneous 3.5 μM methylmercury and selenite treatments indicated protection with increased selenite concentrations. In both exposure protocols, the 5 μM methylmercury treatments were toxic to the cells.

In both study protocols, cells exposed to selenite and mercuric chloride manifested increased cellular mercury uptake with increased selenium concentration. In all selenite and 35 μM mercuric chloride treatments, no inhibition of growth was observed, while the 50 μM mercuric chloride treatments were toxic to the cells. Selenite-dependent protection was achieved in both exposure protocols when considering the cellular uptake of mercury. With few exceptions, selenomethionine produced similar effects as selenite on mercuric chloride uptake and growth inhibition.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 2001. , p. 62
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 988
Keywords [en]
Oncology, growth inhibition, inorganic mercury, interaction, organic mercury, selenite, selenomethionine, toxicity, uptake
Keywords [sv]
Onkologi
National Category
Cancer and Oncology
Research subject
Biomedical Radiation Science
Identifiers
URN: urn:nbn:se:uu:diva-575ISBN: 91-554-4913-1 (print)OAI: oai:DiVA.org:uu-575DiVA, id: diva2:166243
Public defence
2001-02-17, Rudbecksalen, Rudbecklaboratoriet, Uppsala, Uppsala, 09:30
Available from: 2001-01-27 Created: 2001-01-27Bibliographically approved

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CiteExportLink to record
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Cite
Citation style
  • apa
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  • de-DE
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Output format
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