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The tRNALeu (UAA) Intron of Cyanobacteria: Towards Understanding a Genetic Marker
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Physiological Botany.
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The tRNALeu (UAA) intron has been recorded in the plastid genome of many algae and land plants and was the first intron to be discovered in cyanobacteria. In all known cases it interrupts the tRNALeu anticodon loop at a conserved position (U-intron-AA). Cyanobacteria are a diverse group of photosynthetic prokaryotes, some involved in symbiotic associations with a wide range of organisms. The most studied associations are those with plants, where strains of Nostoc are the common cyanobacterial partner. In this thesis two aspects of the biology of the cyanobacterial tRNALeu (UAA) intron are focused: first, the use of the intron as a genetic marker for studying the diversity and specificity of two cyanobacterial symbiosis (bryophytes and cycads) and second, the evolutionary patterns of the intron by using the unique data set generated from the diversity analysis.

From the studies, many different Nostoc strains are involved in the two symbiotic associations, although no variation was observed within a single bryophyte cavity or cycad coralloid root. Furthermore, a certain level of temporal stability in the cyanobiont composition of the bryophyte population was found and, in the cycad association different coralloid roots from a single specimen may harbor different cyanobacteria. That a minor cyanobiont could have avoided detection is still possible but unlikely. The sequence alignment of the Nostoc tRNALeu (UAA) introns reveals great sequence similarity with size variation only found in the structural element P6b. This element was found to consist of heptanucleotide repeats and of other non-repetitive genetic elements (NIS elements). The sporadic occurrence of the NIS elements indicates recent origins and a mechanism for its dispersal is proposed.

In this thesis new insights are given concerning cyanobacterial symbioses and also on the mechanisms involved in the evolution of an old genetic element: the tRNALeu (UAA) intron in cyanobacteria.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 2004. , p. 49
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 1009
Keyword [en]
Biology
Keyword [sv]
Biologi
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-4537ISBN: 91-554-6033-X (print)OAI: oai:DiVA.org:uu-4537DiVA, id: diva2:165074
Public defence
2004-10-15, Lecture Hall, room 3041, Department of Physiological Botany, Villavägen 6, Uppsala, 10:00 (English)
Opponent
Supervisors
Available from: 2004-09-23 Created: 2004-09-23 Last updated: 2009-04-02Bibliographically approved
List of papers
1. Cyanobiont diversity within coralloid roots of selected cycad species
Open this publication in new window or tab >>Cyanobiont diversity within coralloid roots of selected cycad species
1999 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, Vol. 28, p. 85-91Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-92108 (URN)
Available from: 2004-09-23 Created: 2004-09-23 Last updated: 2012-08-22
2. Sequenced based data supports a single Nostoc strain in coralloid roots of cycads
Open this publication in new window or tab >>Sequenced based data supports a single Nostoc strain in coralloid roots of cycads
2004 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, Vol. 49, p. 481-487Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-92109 (URN)
Available from: 2004-09-23 Created: 2004-09-23 Last updated: 2012-08-22
3. Genetic diversity of Nostoc symbionts endophytically associated with two bryophyte species
Open this publication in new window or tab >>Genetic diversity of Nostoc symbionts endophytically associated with two bryophyte species
2001 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 67, p. 4393-4396Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-92110 (URN)
Available from: 2004-09-23 Created: 2004-09-23 Last updated: 2012-08-22
4. The cyanobacterial tRNA(Leu) (UAA) intron: Evolutionary patterns in a genetic marker
Open this publication in new window or tab >>The cyanobacterial tRNA(Leu) (UAA) intron: Evolutionary patterns in a genetic marker
2002 (English)In: Molecular Biology and Evolution, ISSN 0737-4038, Vol. 19, p. 850-857Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-92111 (URN)
Available from: 2004-09-23 Created: 2004-09-23 Last updated: 2012-08-22
5. Dispersal of iterated sequences in the genome of the cyanobacterium Nostoc punctiforme
Open this publication in new window or tab >>Dispersal of iterated sequences in the genome of the cyanobacterium Nostoc punctiforme
(English)Manuscript (Other (popular science, discussion, etc.))
Identifiers
urn:nbn:se:uu:diva-92112 (URN)
Available from: 2004-09-23 Created: 2004-09-23 Last updated: 2010-01-14Bibliographically approved

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