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Biological and Molecular Characteristics of Microorganism-Stimulated Defence Response in Lycopersicon esculentum –L
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Microorganisms, including two fungi, Phytophthora cryptogea and Fusarium oxysporum strain Fo-(IMI 386351), and one bacterium, Pesudomonas sp. strain MF30, were tested for their abilities to stimulate plant defence responses in tomato (Lycopersicon esculentum –L.) and to serve as effective biocontrol agents (Bs). The study included in vivo and in vitro characterization of biological attributes of the microorganisms, pertaining to their abilities to stimulate plant immunity against a fungal pathogen, Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of tomato wilt disease. Using Lycopersicon esculentum –L. as a model plant for examining some fundamental elements of the plant-microorganism interaction, the study reveals and clarifies some aspects of the close association and the complexity of such systems.

For each B, the results revealed a B-distinct plant-microorganism interaction, which included systemic induced resistance (SIR). A phylogenetic analyses of the partial sequences of two Fo-(IMI 386351) genes, a mitochondrial small subunit ribosomal DNA (mtSSU rDNA) and the nuclear translation elongation factor 1α (EF-1α), provided phylogenetic trees confirming that Fo-(IMI 386351) might be a member of Fol or of F. oxysporum f. sp. melonis, which have polyphyletic evolutionary origins. RFLP analysis (mtDNA), suggested that Fo-(IMI 386351) probably belongs to Fol. For routine and accurate differentiation between two morphologically indistinguishable F. oxysporum formae speciales strains, F. oxysporum f. sp. lycopersici and F. oxysporum f. sp. radicis-lycopersici, a molecular method (mtDNA RFLP analysis) was developed, and its usefulness for such differentiation was compared with that of two other methods: isozyme analysis and an osmotic method, revealed with high performance liquid chromatography (HPLC). The HPLC-spectra of Fo-(IMI 386351) had an extra peak for the two tested fractions, indicating that activation of the observed plant defence mechanism could have been at least partially the result of one of the products of the eliciting microbe. Preliminary results obtained by nuclear magnetic resonance spectrometry of those fractions suggest that the extra peak probably represents an oligosaccharide, which may have acted as a mobile signal and triggered the plant defence mechanisms.

We concluded that (1) our three tested microorganisms are able to stimulate plant defence mechanisms by triggering SIR (plant immunity), (2) the complexity and elaborateness of evolved plant-microbe interactions involving plant defence can, at least in some cases, be observed and studied in the laboratory, and (3) molecular tools can be a powerful means for identifying fungal strains and for clarifying their taxonomical relationships.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 2004. , p. 82
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 943
Keywords [en]
Biology, Phytophthora cryptogea, Fusarium oxysporum strain IMI 386351, Pseudomonas sp. strain MF30, plant defence, phylogenetic analysis, differentiation methods
Keywords [sv]
Biologi
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-4012ISBN: 91-554-5888-2 (print)OAI: oai:DiVA.org:uu-4012DiVA, id: diva2:164132
Public defence
2004-03-12, Lindahlsalen, EBC, Department of Molecular Evolution, Norbyvägen 18C, Uppsala University, SE-752 36, Uppsala, 10:00
Opponent
Supervisors
Available from: 2004-02-19 Created: 2004-02-19 Last updated: 2011-03-04Bibliographically approved
List of papers
1. A rapid molecular method for differentiating two special forms (lycopersici and radicis-lycopersici) of Fusarium oxysporum
Open this publication in new window or tab >>A rapid molecular method for differentiating two special forms (lycopersici and radicis-lycopersici) of Fusarium oxysporum
2004 (English)In: Mycological Research, ISSN 0953-7562, E-ISSN 1469-8102, Vol. 108, no 7, p. 787-794Article in journal (Refereed) Published
Abstract [en]

Two pathogenic special forms (f. sp.) of the Fusarium oxysporum species complex f. sp. lycopersici (Fol) and f. sp. radicis-lycopersici (Forl) are morphologically indistinguishable. Although they are pathogenic to the same host genus Lycopersicon (tomato), and infect the same tomato cultivar, they form distinct diseases; Fol causes wilt and Forl causes crown rot and root rot. These two special forms apparently exist as genetically isolated populations, based on vegetative compatibility and molecular variation at the DNA level. In seeking efficient diagnostic tools for differentiating Fol and Forl isolates, we examined three techniques: isozyme analysis, mitochondrial DNA (mtDNA) RFLP by HaeIII-digestion of total genomic DNA, and an osmotic method using high performance liquid chromatography (HPLC) to detect fungal pigments. The isolates were collected from geographically widespread locations. Distinct HPLC-profile differences were found between an endophytic non-pathogenic isolate and the other pathogenic isolates. However, the direct mtDNA RFLP technique proved to be an efficient diagnostic tool for routine differentiation of Fol and Forl isolates.

National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-91409 (URN)10.1017/S0953756204000322 (DOI)
Available from: 2004-02-19 Created: 2004-02-19 Last updated: 2017-12-14Bibliographically approved
2. Biology and partial sequencing of an endophytic Fusarium oxysporum and plant defense complex
Open this publication in new window or tab >>Biology and partial sequencing of an endophytic Fusarium oxysporum and plant defense complex
Manuscript (Other academic)
Identifiers
urn:nbn:se:uu:diva-91410 (URN)
Available from: 2004-02-19 Created: 2004-02-19 Last updated: 2010-01-13Bibliographically approved
3. Systemic resistance to Fusarium wilt in tomato induced by Phytophthora cryptogea
Open this publication in new window or tab >>Systemic resistance to Fusarium wilt in tomato induced by Phytophthora cryptogea
2001 In: Journal of Phytopathology, ISSN 0931-1785, Vol. 149, no 7-8, p. 373-380Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-91411 (URN)
Available from: 2004-02-19 Created: 2004-02-19Bibliographically approved
4. Pseudomonas sp. strain MF30 suppresses Fusarium wilt of tomato in vivo
Open this publication in new window or tab >>Pseudomonas sp. strain MF30 suppresses Fusarium wilt of tomato in vivo
2001 In: Phytopathologia Mediterranea, ISSN 0031-9465, Vol. 40, no 3, p. 234-239Article in journal (Refereed) Published
Identifiers
urn:nbn:se:uu:diva-91412 (URN)
Available from: 2004-02-19 Created: 2004-02-19Bibliographically approved

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