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PDMS leaching and its implications for on-chip studies focusing on boneregeneration applications
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Microsystems Technology. (EMBLA)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Materials Science and Engineering, Microsystems Technology. (EMBLA)ORCID iD: 0000-0002-5645-8323
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Lanekoff Group)ORCID iD: 0000-0001-9040-3230
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2020 (English)In: Organs-on-a-Chip, ISSN 2666-1020, Vol. 2, no 100004Article in journal (Refereed) Published
Abstract [en]

Polydimethylsiloxane (PDMS) is among the most widely used materials for organ-on-chip systems. Despite itsmultiple beneficial characteristics from an engineering point of view, there is a concern about the effect of PDMSon the cells cultured in such devices. The aim of this study was to enhance the understanding of the effect of PDMSon cellular behavior in a context relevant for on-chip studies. The focus was put on an indirect effect of PDMS,namely leaching of uncrosslinked oligomers, particularly for bone regeneration applications. PDMS-based chipswere prepared and analyzed for the potential release of PDMS oligomers within the microfluidic channel whenkept at different flow rates. Leaching of uncrosslinked oligomers from PDMS was quantified as silicon concen-tration by inductively coupled plasma - optical emission spectrometry and further confirmed by mass spec-trometry. Subsequently, PDMS-leached media, with a silicon concentration matching the on-chip experiment,were prepared to study cell proliferation and osteogenic differentiation of MC3T3-E1 pre-osteoblasts and humanmesenchymal stem cells. The silicon concentration initially detected in the media was inversely proportional tothe tested flow rates and decreased to control levels within 52 h. In addition, by curing the material overnightinstead of 2 h, regardless of the curing temperature (65 and 80 C), a large reduction in silicon concentration wasfound, indicating the importance of the PDMS curing parameters. Furthermore, it was shown that PDMS oligo-mers enhanced the differentiation of MC3T3-E1 pre-osteoblasts, this being a cell type dependent effect as nochanges in cell differentiation were observed for human mesenchymal stem cells. Overall, this study illustrates theimportance of optimization steps when using PDMS devices for biological studies, in particular PDMS curingconditions and extensive washing steps prior to an experiment.

Place, publisher, year, edition, pages
Elsevier, 2020. Vol. 2, no 100004
Keywords [en]
PDMS, Organs-on-chip, Human mesenchymal stem cells, Osteoblasts, Silicon
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-410262DOI: 10.1016/j.ooc.2020.100004OAI: oai:DiVA.org:uu-410262DiVA, id: diva2:1430172
Funder
Swedish Research Council Formas, 2016-00781Swedish Research Council, 2017-05051Göran Gustafsson Foundation for Research in Natural Sciences and Medicine, 1841Knut and Alice Wallenberg Foundation, 2016-0112Available from: 2020-05-13 Created: 2020-05-13 Last updated: 2020-05-15Bibliographically approved

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Carter, Sarah-SophiaAtif, Abdul RaoufKadekar, SandeepLanekoff, IngelaEngqvist, HåkanVarghese, Oommen P.Tenje, MariaMestres, Gemma
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