Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Mucosal Metabolomic Profiling and Pathway Analysis Reveal the Metabolic Signature of Ulcerative Colitis
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
Show others and affiliations
2019 (English)In: Metabolites, ISSN 2218-1989, E-ISSN 2218-1989, Vol. 9, no 12, article id 291Article in journal (Refereed) Published
Abstract [en]

The onset of ulcerative colitis (UC) is characterized by a dysregulated mucosal immune response triggered by several genetic and environmental factors in the context of host–microbe interaction. This complexity makes UC ideal for metabolomic studies to unravel the disease pathobiology and to improve the patient stratification strategies. This study aims to explore the mucosal metabolomic profile in UC patients, and to define the UC metabolic signature. Treatment- naïve UC patients (n = 18), UC patients in deep remission (n = 10), and healthy volunteers (n = 14) were recruited. Mucosa biopsies were collected during colonoscopies. Metabolomic analysis was performed by combined gas chromatography coupled to time-of-flight mass spectrometry (GC-TOF-MS) and ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS). In total, 177 metabolites from 50 metabolic pathways were identified. The most prominent metabolome changes among the study groups were in lysophosphatidylcholine, acyl carnitine, and amino acid profiles. Several pathways were found perturbed according to the integrated pathway analysis. These pathways ranged from amino acid metabolism (such as tryptophan metabolism) to fatty acid metabolism, namely linoleic and butyrate. These metabolic changes during UC reflect the homeostatic disturbance in the gut, and highlight the importance of system biology approaches to identify key drivers of pathogenesis which prerequisite personalized medicine.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 9, no 12, article id 291
Keywords [en]
inflammatory bowel disease, metabolomics, pathway analysis, ulcerative colitis, tryptophan metabolism, fatty acid metabolism, personalized treatment
National Category
Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:umu:diva-167640DOI: 10.3390/metabo9120291ISI: 000506676300010PubMedID: 31783598Scopus ID: 2-s2.0-85076503177OAI: oai:DiVA.org:umu-167640DiVA, id: diva2:1391013
Available from: 2020-02-03 Created: 2020-02-03 Last updated: 2020-02-03Bibliographically approved

Open Access in DiVA

fulltext(2224 kB)13 downloads
File information
File name FULLTEXT01.pdfFile size 2224 kBChecksum SHA-512
87987f20eac8ae63491dbf74aa0e5c5f8c0e5c2698735a15e18d957bde60062ba469a2236f24d1eb78ffd1f9cd6c2975e77790a46176e0e4f5ba0945bcfa9d56
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Diab, JosephStenlund, HansMoritz, Thomas
By organisation
Department of Molecular Biology (Faculty of Science and Technology)Department of Molecular Biology (Faculty of Medicine)
In the same journal
Metabolites
Gastroenterology and Hepatology

Search outside of DiVA

GoogleGoogle Scholar
Total: 13 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 20 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf