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Characterization of the transient fluorescence wave phenomenon that occurs during H-2 production in Chlamydomonas reinhardtii
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. John Innes Ctr, Dept Mol Microbiol, Norwich NR4 7UH, Norfolk, England.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. Univ Oxford, Dept Chem, Inorgan Chem Lab, Oxford OX1 3QR, England.ORCID iD: 0000-0003-3298-9194
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.ORCID iD: 0000-0002-6218-3039
2019 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 70, no 21, p. 6321-6336Article in journal (Refereed) Published
Abstract [en]

The redox state of the plastoquinone (PQ) pool in sulfur-deprived, H-2-producing Chlamydomonas reinhardtii cells was studied using single flash-induced variable fluorescence decay kinetics. During H-2 production, the fluorescence decay kinetics exhibited an unusual post-illumination rise of variable fluorescence, giving a wave-like appearance. The wave showed the transient fluorescence minimum at 60 ms after the flash, followed by a rise, reaching the transient fluorescence maximum at 1 s after the flash, before decaying back to the initial fluorescence level. Similar wave-like fluorescence decay kinetics have been reported previously in anaerobically incubated cyanobacteria but not in green algae. From several different electron and proton transfer inhibitors used, polymyxin B, an inhibitor of type II NAD(P)H dehydrogenase (NDA2), had the effect of eliminating the fluorescence wave feature, indicating involvement of NDA2 in this phenomenon. This was further confirmed by the absence of the fluorescence wave in the Delta nda2 mutant lacking NDA2. Additionally, Delta nda2 mutants have also shown delayed and diminished H-2 production (only 23% if compared with the wild type). Our results show that the fluorescence wave phenomenon in C. reinhardtii is observed under highly reducing conditions and is induced by the NDA2-mediated electron flow from the reduced stromal components to the PQ pool. Therefore, the fluorescence wave phenomenon is a sensitive probe for the complex network of redox reactions at the PQ pool level in the thylakoid membrane. It could be used in further characterization and improvement of the electron transfer pathways leading to H-2 production in C. reinhardtii.

Place, publisher, year, edition, pages
OXFORD UNIV PRESS , 2019. Vol. 70, no 21, p. 6321-6336
Keywords [en]
Chlamydomonas reinhardtii, hydrogen production, plastoquinone pool, sulfur deprivation, type II NDH, variable fluorescence
National Category
Botany
Identifiers
URN: urn:nbn:se:uu:diva-402225DOI: 10.1093/jxb/erz380ISI: 000503054400025PubMedID: 31504725OAI: oai:DiVA.org:uu-402225DiVA, id: diva2:1386447
Available from: 2020-01-17 Created: 2020-01-17 Last updated: 2020-01-17Bibliographically approved

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