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Meta-Transcriptomic Comparison of the RNA Viromes of the Mosquito Vectors Culex pipiens and Culex torrentium in Northern Europe
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Univ Sydney, Charles Perkins Ctr, Sch Life & Environm Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW 2006, Australia;Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia.ORCID iD: 0000-0001-6665-7492
Univ Sydney, Charles Perkins Ctr, Sch Life & Environm Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW 2006, Australia;Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia.
Univ Sydney, Charles Perkins Ctr, Sch Life & Environm Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW 2006, Australia;Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia;Westmead Inst Med Res, Ctr Virus Res, Sydney, NSW 2145, Australia.
Univ Sydney, Charles Perkins Ctr, Sch Life & Environm Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW 2006, Australia;Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia.
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2019 (English)In: Viruses, ISSN 1999-4915, E-ISSN 1999-4915, Vol. 11, no 11, article id 1033Article in journal (Refereed) Published
Abstract [en]

Mosquitoes harbor an extensive diversity of 'insect-specific' RNA viruses in addition to those important to human and animal health. However, because most studies of the mosquito virome have been conducted at lower latitudes, little is known about the diversity and evolutionary history of RNA viruses sampled from mosquitoes in northerly regions. Here, we compared the RNA virome of two common northern mosquito species, Culex pipiens and Culex torrentium, collected in south-central Sweden. Following bulk RNA-sequencing (meta-transcriptomics) of 12 libraries, comprising 120 specimens of Cx. pipiens and 150 specimens of Cx. torrentium, we identified 40 viruses (representing 14 virus families) of which 28 were novel based on phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) protein. Hence, we documented similar levels of virome diversity as in mosquitoes sampled from the more biodiverse lower latitudes. Many viruses were also related to those sampled on other continents, indicative of a widespread global movement and/or long host-virus co-evolution. Although the two mosquito species investigated have overlapping geographical distributions and share many viruses, several viruses were only found at a specific location at this scale of sampling, such that local habitat and geography may play an important role in shaping viral diversity in Culex mosquitoes.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 11, no 11, article id 1033
Keywords [en]
mosquito-borne RNA viruses, evolution, ecology, meta-transcriptomics, RNA-sequencing, Culex mosquitoes
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-400736DOI: 10.3390/v11111033ISI: 000502292300061PubMedID: 31698792OAI: oai:DiVA.org:uu-400736DiVA, id: diva2:1386436
Funder
Swedish Research Council Formas, 2015-710Available from: 2020-01-17 Created: 2020-01-17 Last updated: 2020-01-17Bibliographically approved

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