Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
29-Color Flow Cytometry: Unraveling Human Liver NK Cell Repertoire Diversity
Karolinska Inst, Karolinska Univ Hosp, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden.ORCID iD: 0000-0002-8166-5500
Karolinska Inst, Karolinska Univ Hosp, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden;Karolinska Inst, Dept Clin Sci Intervent & Technol, Div Transplantat Surg, Stockholm, Sweden.
Karolinska Inst, Karolinska Univ Hosp, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.ORCID iD: 0000-0001-8327-1292
Show others and affiliations
2019 (English)In: Frontiers in Immunology, ISSN 1664-3224, E-ISSN 1664-3224, Vol. 10, article id 2692Article in journal (Refereed) Published
Abstract [en]

Recent studies have demonstrated extraordinary diversity in peripheral blood human natural killer (NK) cells and have suggested environmental control of receptor expression patterns on distinct subsets of NK cells. However, tissue localization may influence NK cell differentiation to an even higher extent and less is known about the receptor repertoire of human tissue-resident NK cells. Advances in single-cell technologies have allowed higher resolution studies of these cells. Here, the power of high-dimensional flow cytometry was harnessed to unravel the complexity of NK cell repertoire diversity in liver since recent studies had indicated high heterogeneity within liver NK cells. A 29-color flow cytometry panel allowing simultaneous measurement of surface tissue-residency markers, activating and inhibitory receptors, differentiation markers, chemokine receptors, and transcription factors was established. This panel was applied to lymphocytes across three tissues (liver, peripheral blood, and tonsil) with different distribution of distinct NK cell subsets. Dimensionality reduction of this data ordered events according to their lineage, rather than tissue of origin. Notably, narrowing the scope of the analysis to the NK cell lineage in liver and peripheral blood separated subsets according to tissue, enabling phenotypic characterization of NK cell subpopulations in individual tissues. Such dimensionality reduction, coupled with a clustering algorithm, identified CD49e as the preferred marker for future studies of liver-resident NK cell subsets. We present a robust approach for diversity profiling of tissue-resident NK cells that can be applied in various homeostatic and pathological conditions such as reproduction, infection, and cancer.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA , 2019. Vol. 10, article id 2692
Keywords [en]
natural killer cells, liver immunology, tissue-resident cells, high-dimensional, flow cytometry
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-400662DOI: 10.3389/fimmu.2019.02692ISI: 000501350100001PubMedID: 31798596OAI: oai:DiVA.org:uu-400662DiVA, id: diva2:1382433
Funder
Swedish Research CouncilSwedish Cancer SocietySwedish Foundation for Strategic Research Knut and Alice Wallenberg FoundationNovo NordiskWenner-Gren FoundationsAvailable from: 2020-01-03 Created: 2020-01-03 Last updated: 2020-01-03Bibliographically approved

Open Access in DiVA

fulltext(2131 kB)6 downloads
File information
File name FULLTEXT01.pdfFile size 2131 kBChecksum SHA-512
40ce29a1cc8c2ad4362217b39c8f40ee69930de4de25bdd43414b6dc6980e30dade09d0cf5cf26e643c2d39c41009d6214d997837071296fd64e21c12363550a
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Filipovic, IvaFriberg, Danielle
By organisation
Otolaryngology and Head and Neck Surgery
In the same journal
Frontiers in Immunology
Immunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 6 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 7 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf