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Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Czech Academy of Sciences.
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2019 (English)In: Cells, ISSN 2073-4409, Vol. 8, article id 1645Article in journal (Refereed) Published
Abstract [en]

Focal adhesion kinase (FAK) is essential for vascular endothelial growth factor-A (VEGFA)/VEGF receptor-2 (VEGFR2)-stimulated angiogenesis and vascular permeability. We have previously noted that presence of the Src homology-2 domain adapter protein B (SHB) is of relevance for VEGFA-stimulated angiogenesis in a FAK-dependent manner. The current study was conducted in order address the temporal dynamics of co-localization between these components in HEK293 and primary lung endothelial cells (EC) by total internal reflection fluorescence microscopy (TIRF). An early (<2.5 min) VEGFA-induced increase in VEGFR2 co-localization with SHB was dependent on tyrosine 1175 in VEGFR2. VEGFA also enhanced SHB co-localization with FAK. FAK co-localization with VEGFR2 was dependent on SHB since it was significantly lower in SHB deficient EC after VEGFA addition. Absence of SHB also resulted in a gradual decline of VEGFR2 co-localization with FAK under basal (prior to VEGFA addition) conditions. A similar basal response was observed with expression of the Y1175F-VEGFR2 mutant in wild type EC. The distribution of focal adhesions in SHB-deficient EC was altered with a primarily perinuclear location. These live cell data implicate SHB as a key component regulating FAK activity in response to VEGFA/VEGFR2.

Place, publisher, year, edition, pages
Basel, Switzerland: MDPI, 2019. Vol. 8, article id 1645
Keywords [en]
VEGFR2; FAK; SHB; TIRF; focal adhesions; angiogenesis
National Category
Basic Medicine
Research subject
Molecular Cellbiology
Identifiers
URN: urn:nbn:se:uu:diva-400532DOI: 10.3390/cells8121645OAI: oai:DiVA.org:uu-400532DiVA, id: diva2:1381576
Available from: 2019-12-23 Created: 2019-12-23 Last updated: 2020-01-03Bibliographically approved

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Pietilä, IlkkaClaesson-Welsh, LenaTengholm, AndersWelsh, Michael
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Department of Medical Cell BiologyDepartment of Immunology, Genetics and Pathology
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