Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Turning Up the Heat: Local Temperature Control During in vivo Imaging of Immune Cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrative Physiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrative Physiology.ORCID iD: 0000-0002-6381-2968
Show others and affiliations
2019 (English)In: Frontiers in Immunology, ISSN 1664-3224, E-ISSN 1664-3224, Vol. 10, article id 2036Article in journal (Refereed) Published
Abstract [en]

Intravital imaging is an invaluable tool for studying the expanding range of immune cell functions. Only in vivo can the complex and dynamic behavior of leukocytes and their interactions with their natural microenvironment be observed and quantified. While the capabilities of high-speed, high-resolution confocal and multiphoton microscopes are well-documented and steadily improving, other crucial hardware required for intravital imaging is often developed in-house and less commonly published in detail. In this report, we describe a low-cost, multipurpose, and tissue-stabilizing in vivo imaging platform that enables sensing and regulation of local tissue temperature. The effect of tissue temperature on local blood flow and leukocyte migration is demonstrated in muscle and skin. Two different models of vacuum windows are described in this report, however, the design of the vacuum window can easily be adapted to fit different organs and tissues.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA , 2019. Vol. 10, article id 2036
Keywords [en]
intravital microscopy, skin, blood flow, leukocytes, vacuum window, confocal microscopy
National Category
Immunology Immunology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-398848DOI: 10.3389/fimmu.2019.02036ISI: 000482819900001PubMedID: 31507619OAI: oai:DiVA.org:uu-398848DiVA, id: diva2:1380241
Funder
Swedish Research Council, 2018-02552Knut and Alice Wallenberg FoundationRagnar Söderbergs stiftelseSwedish Society for Medical Research (SSMF)Göran Gustafsson Foundation for promotion of scientific research at Uppala University and Royal Institute of TechnologyNovo NordiskAvailable from: 2019-12-18 Created: 2019-12-18 Last updated: 2019-12-18Bibliographically approved

Open Access in DiVA

fulltext(3949 kB)8 downloads
File information
File name FULLTEXT01.pdfFile size 3949 kBChecksum SHA-512
3ceec7d6077eb2f163e0445f9106052ee74d5cadd8cb659d2264630d50103173ebcf0ca53bee0464267e9efa56bcff1c9fb63647c030ca365655c7c5b13c7c85
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ahl, DavidEriksson, OlleSedin, JohnSeignez, CedricKreuger, JohanChristoffersson, GustafPhillipson, Mia
By organisation
Integrative PhysiologyDepartment of Medical Cell Biology
In the same journal
Frontiers in Immunology
ImmunologyImmunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 8 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 19 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf