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Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.ORCID iD: 0000-0001-6665-7492
UVE Aix Marseille Univ, Inserm 1207, IRD 190, IHU Mediterranee Infect,Unite Virus Emergents, Marseille, France.
Mahosot Hosp, Lao Oxford Mahosot Hosp, Microbiol Lab, Wellcome Trust Res Unit LOMWRU, Viangchan, Laos;Univ Hlth Sci, IRED, Viangchan, Laos;Univ Oxford, Ctr Trop Med & Global Hlth, Churchill Hosp, Nuffield Dept Med, Oxford, England.
Mahosot Hosp, Lao Oxford Mahosot Hosp, Microbiol Lab, Wellcome Trust Res Unit LOMWRU, Viangchan, Laos.
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2019 (English)In: Diagnostic microbiology and infectious disease, ISSN 0732-8893, E-ISSN 1879-0070, Vol. 95, no 4, article id UNSP 114878Article in journal (Refereed) Published
Abstract [en]

Determining the etiological basis of central nervous system (CNS) infections is inherently challenging, primarily due to the multi-etiological nature. Using RNA sequencing, we aimed to identify microbes present in cerebrospinal fluid (CSF) of two patients suffering CNS infection, previously diagnosed with Cryptococcus sp. and Streptococcus pneumoniae infection, respectively. After meta-transcriptomic analysis, and confirmation with real-time PCR, hepatitis B virus (HBV) was detected in the CSF of two patients diagnosed with CNS syndrome. Phylogenetic analysis of the partial HBV genomes from these patients showed that they belonged to genotypes B and C and clustered with other viruses of Asian origin. In countries with high levels of HBV endemicity, the virus is likely to be found in patients diagnosed with CNS infections, although whether it contributes to symptoms and pathology, or is simply a coincidental infection, is unknown and merits further investigation. (C) 2019 The Authors. Published by Elsevier Inc.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC , 2019. Vol. 95, no 4, article id UNSP 114878
Keywords [en]
Hepatitis B virus, RNA sequencing, Meta-transcriptomics, Cerebrospinal fluid, Encephalitis
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:uu:diva-400049DOI: 10.1016/j.diagmicrobio.2019.114878ISI: 000498755300010PubMedID: 31451314OAI: oai:DiVA.org:uu-400049DiVA, id: diva2:1380091
Funder
Swedish Research Council Formas, 2015-710EU, Horizon 2020, 653316Available from: 2019-12-18 Created: 2019-12-18 Last updated: 2019-12-18Bibliographically approved

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