Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A tetracycline-repressible transactivator system to study essential genes in malaria parasites
Show others and affiliations
2012 (English)In: Cell Host and Microbe, ISSN 1931-3128, E-ISSN 1934-6069, Vol. 12, no 6, p. 824-834Article in journal (Refereed) Published
Abstract [en]

A major obstacle in analyzing gene function in apicomplexan parasites is the absence of a practical regulatable expression system. Here, we identified functional transcriptional activation domains within Apicomplexan AP2 (ApiAP2) family transcription factors. These ApiAP2 transactivation domains were validated in blood-, liver-, and mosquito-stage parasites and used to create a robust conditional expression system for stage-specific, tetracycline-dependent gene regulation in Toxoplasma gondii, Plasmodium berghei, and Plasmodium falciparum. To demonstrate the utility of this system, we created conditional knockdowns of two essential P. berghei genes: profilin (PRF), a protein implicated in parasite invasion, and N-myristoyltransferase (NMT), which catalyzes protein acylation. Tetracycline-induced repression of PRF and NMT expression resulted in a dramatic reduction in parasite viability. This efficient regulatable system will allow for the functional characterization of essential proteins that are found in these important parasites.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 12, no 6, p. 824-834
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-165864DOI: 10.1016/j.chom.2012.10.016ISI: 000313406600013PubMedID: 23245327OAI: oai:DiVA.org:umu-165864DiVA, id: diva2:1379124
Funder
Wellcome trust, WT098051Wellcome trust, WT077502MAAvailable from: 2019-12-16 Created: 2019-12-16 Last updated: 2019-12-18Bibliographically approved

Open Access in DiVA

fulltext(1773 kB)6 downloads
File information
File name FULLTEXT01.pdfFile size 1773 kBChecksum SHA-512
af4ee8f318e225713448c11e8142f2303efafde865b13658b412dc0173c6f1865fac07d060e954c640de3dc315bbe945399cc423d10691c0662fb3d4739161ae
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Billker, Oliver
In the same journal
Cell Host and Microbe
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 6 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 9 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf