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Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates
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2013 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 27, no 10, p. 1198-1215Article in journal (Refereed) Published
Abstract [en]

Fertilization is a crucial yet poorly characterized event in eukaryotes. Our previous discovery that the broadly conserved protein HAP2 (GCS1) functioned in gamete membrane fusion in the unicellular green alga Chlamydomonas and the malaria pathogen Plasmodium led us to exploit the rare biological phenomenon of isogamy in Chlamydomonas in a comparative transcriptomics strategy to uncover additional conserved sexual reproduction genes. All previously identified Chlamydomonas fertilization-essential genes fell into related clusters based on their expression patterns. Out of several conserved genes in a minus gamete cluster, we focused on Cre06.g280600, an ortholog of the fertilization-related Arabidopsis GEX1. Gene disruption, cell biological, and immunolocalization studies show that CrGEX1 functions in nuclear fusion in Chlamydomonas. Moreover, CrGEX1 and its Plasmodium ortholog, PBANKA_113980, are essential for production of viable meiotic progeny in both organisms and thus for mosquito transmission of malaria. Remarkably, we discovered that the genes are members of a large, previously unrecognized family whose first-characterized member, KAR5, is essential for nuclear fusion during yeast sexual reproduction. Our comparative transcriptomics approach provides a new resource for studying sexual development and demonstrates that exploiting the data can lead to the discovery of novel biology that is conserved across distant taxa.

Place, publisher, year, edition, pages
Cold Spring Harbor Laboratory Press (CSHL), 2013. Vol. 27, no 10, p. 1198-1215
Keywords [en]
RNA sequencing; fertilization; Plasmodium; Chlamydomonas; KAR5/GEX1/Brambleberry; nuclear envelope fusion
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Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-165862DOI: 10.1101/gad.212746.112ISI: 000319393800010PubMedID: 23699412OAI: oai:DiVA.org:umu-165862DiVA, id: diva2:1379088
Funder
NIH (National Institute of Health), C06 RR 30414NIH (National Institute of Health), GM56778NIH (National Institute of Health), GM25661Wellcome trust, 098051Available from: 2019-12-16 Created: 2019-12-16 Last updated: 2019-12-18Bibliographically approved

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