Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
PlasmoGEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites
Show others and affiliations
2015 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 43, no Database issue, p. D1176-D1182Article in journal (Refereed) Published
Abstract [en]

The Plasmodium Genetic Modification (PlasmoGEM) database (http://plasmogem.sanger.ac.uk) provides access to a resource of modular, versatile and adaptable vectors for genome modification of Plasmodium spp. parasites. PlasmoGEM currently consists of >2000 plasmids designed to modify the genome of Plasmodium berghei, a malaria parasite of rodents, which can be requested by non-profit research organisations free of charge. PlasmoGEM vectors are designed with long homology arms for efficient genome integration and carry gene specific barcodes to identify individual mutants. They can be used for a wide array of applications, including protein localisation, gene interaction studies and high-throughput genetic screens. The vector production pipeline is supported by a custom software suite that automates both the vector design process and quality control by full-length sequencing of the finished vectors. The PlasmoGEM web interface allows users to search a database of finished knock-out and gene tagging vectors, view details of their designs, download vector sequence in different formats and view available quality control data as well as suggested genotyping strategies. We also make gDNA library clones and intermediate vectors available for researchers to produce vectors for themselves.

Place, publisher, year, edition, pages
Oxford University Press, 2015. Vol. 43, no Database issue, p. D1176-D1182
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-165854DOI: 10.1093/nar/gku1143ISI: 000350210400172PubMedID: 25593348OAI: oai:DiVA.org:umu-165854DiVA, id: diva2:1379087
Funder
Wellcome trust, 089085/Z/09/ZAvailable from: 2019-12-16 Created: 2019-12-16 Last updated: 2019-12-18Bibliographically approved

Open Access in DiVA

fulltext(4036 kB)12 downloads
File information
File name FULLTEXT01.pdfFile size 4036 kBChecksum SHA-512
5761a5b9a48dbd4a60ef82d6f35a22b65af805d6f7ddba858ffbeddb817c928cff74ccab4c02e010d38b7135c0b7dcfe7b1a73bbd5d3f40c4531d86dff1a5118
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Billker, Oliver
In the same journal
Nucleic Acids Research
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 12 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 11 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf