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On-chip background dilution in droplets with high particle recovery using acoustophoresis
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0001-7960-5878
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0001-7980-376x
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0003-4475-6478
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.ORCID iD: 0000-0003-2744-1634
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2019 (English)In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 13, article id 064123Article in journal (Refereed) Published
Abstract [en]

Droplet microfluidics has shown great potential for on-chip biological and chemical assays. However, fluid exchange in droplet microfluidics with high particle recovery is still a major bottleneck. Here, using acoustophoresis, we present for the first time a label-free method to achieve continuous background dilution in droplets containing cells with high sample recovery. The system comprises droplet generation, acoustic focusing, droplet splitting, picoinjection, and serpentine mixing on the same chip. The capacities of the picoinjection and the droplet split to dilute the background fluorescent signal in the droplets have been characterized. The sample recovery at different droplet split ratios has also been characterized. The results show a maximum of 4.3-fold background dilution with 87.7% particle recovery. We also demonstrated that the system can be used to dilute background fluorescent signal in droplets containing either polystyrene particles or endothelial cells.

Place, publisher, year, edition, pages
American Institute of Physics (AIP), 2019. Vol. 13, article id 064123
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-398535DOI: 10.1063/1.5129256OAI: oai:DiVA.org:uu-398535DiVA, id: diva2:1376048
Funder
Swedish Research CouncilAvailable from: 2019-12-06 Created: 2019-12-06 Last updated: 2019-12-12Bibliographically approved

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Liu, ZhenhuaFornell, AnnaBarbe, LaurentHjort, KlasTenje, Maria
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