Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Normal mammary gland development after MMTV-Cre mediated conditional PAK4 gene depletion
Karolinska Inst, Dept Biosci & Nutr, Stockholm, Sweden.
Karolinska Inst, Dept Biosci & Nutr, Stockholm, Sweden;Xinxiang Med Univ, Henan Collaborat Innovat Ctr Mol Diag & Lab Med, Sch Lab Med, Xinxiang, Henan, Peoples R China.
Karolinska Inst, Dept Biosci & Nutr, Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Neuro-Oncology. Karolinska Inst, Dept Biosci & Nutr, Stockholm, Sweden.
Show others and affiliations
2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 14436Article in journal (Refereed) Published
Abstract [en]

p21-activated kinases (PAKs) are serine/threonine kinases functioning as downstream effectors of the small GTPases Rac1 and Cdc42. Members of the PAK family are overexpressed in human breast cancer, but their role in mammary gland development is not fully explored. Here we examined the functional role of PAK4 in mammary gland development by creating a mouse model of MMTV-Cre driven conditional PAK4 gene depletion in the mammary gland. The PAK4 conditional knock-out mice were born healthy, with no observed developmental deficits. Mammary gland whole-mounts revealed no defects in ductal formation or elongation of the mammary tree through the fat pad. PAK4 gene depletion also did not alter proliferation and invasion of the mammary epithelium in young virgin mice. Moreover, adult mice gave birth to healthy pups with normal body weight upon weaning. This implies that MMTV-Cre induced gene depletion of PAK4 in mice does not impair normal mammary gland development and thereby provides an in vivo model that can be explored for examination of the potential function of PAK4 in breast cancer.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 9, article id 14436
National Category
Cancer and Oncology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-396723DOI: 10.1038/s41598-019-50819-4ISI: 000489099500032PubMedID: 31594963OAI: oai:DiVA.org:uu-396723DiVA, id: diva2:1375904
Funder
Swedish Cancer SocietySwedish Research CouncilKnut and Alice Wallenberg FoundationAvailable from: 2019-12-06 Created: 2019-12-06 Last updated: 2019-12-12Bibliographically approved

Open Access in DiVA

fulltext(4123 kB)17 downloads
File information
File name FULLTEXT01.pdfFile size 4123 kBChecksum SHA-512
7ff1626b47cbb3ade1051619faaed05b11f585811a6609c1a10fcafb8bbd920af1534c6fa59034725e560a51677e348a1388d874a427725106b2c39c74bf795c
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Zhao, MiaoStrömblad, Staffan
By organisation
Neuro-Oncology
In the same journal
Scientific Reports
Cancer and OncologyMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 17 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 18 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf