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Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant
Division of Quality and Sensory of Plant Products, University of Göttingen, Göttingen, Germany.
bDepartment of Plant Biology, University of Pécs, Pécs, Hungary.
Department of Plant Biology, University of Pécs, Pécs, Hungary.
Department of Plant Quality and Food Security, Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany.
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2020 (English)In: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 19, p. 217-228Article in journal (Refereed) Published
Abstract [en]

Pyridoxine (vitamin B6) and its vitamers are used by living organisms both as enzymatic cofactors and as antioxidants. We used Arabidopsis pyridoxine biosynthesis mutant pdx1.3-1to study involvement of the PLP-synthase main polypeptide PDX1 in plant responses to ultraviolet radiation of two different qualities, one containing primarily UV-A (315-400 nm), the other containing both UV-A and UV-B (280-315 nm). The antioxidant capacity and the flavonoid and glucosinolate (GS) profiles were examined. As indicator of stress, F⁠v/F⁠mof photosystem II reaction centers was used. In pdx1.3-1, UV-A+B exposure led to a significant 5% decrease in F⁠v/F⁠mon the last day (day 15), indicating mild stress at this time point. Antioxidant capacity of Col-0 wildtype increased significantly (50-73%) after 1 and 3 days of UV-A+B. Instead, in pdx1.3-1, the antioxidant capacity significantly decreased by 44-52% over the same time period, proving the importance of a full complement of functional PDX1genes for detoxification of reactive oxygen species. There were no significant changes in flavonoid glycoside profile under any light condition. However, the GS profile was significantly altered, both with respect to Arabidopsis accession and exposure to UV. The difference in flavonoid and GS profiles reflect that the GS biosynthesis pathway contains at least one pyridoxine-dependent enzyme, whereas no such enzyme is used in flavonoid biosynthesis. Also, there was strong correlation between the antioxidant capacity and the content of some GS compounds. Our results show that vitamin B6vitamers, functioning both as antioxidants and co-factors, are of importance for physiological fitness of plants.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2020. Vol. 19, p. 217-228
National Category
Botany Biochemistry and Molecular Biology Analytical Chemistry
Research subject
Biochemistry
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URN: urn:nbn:se:oru:diva-78129DOI: 10.1039/C9PP00342HPubMedID: 31961357OAI: oai:DiVA.org:oru-78129DiVA, id: diva2:1372968
Projects
UV4quality
Funder
Swedish Research Council Formas, 942-2015-516Knowledge Foundation, 20130164Available from: 2019-11-25 Created: 2019-11-25 Last updated: 2020-02-19Bibliographically approved

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Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1 mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant(2121 kB)22 downloads
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