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Kinetic studies of carrier conjugated protease inhibitors
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC. (Johansson group)
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Conjugates of soybean trypsin inhibitor (SBTI) and potato serine protease inhibitor (PSPI) immobilized on metal oxide particles of ~100nm diameter were prepared. Inhibition of trypsin hydrolysis of BAPA by these conjugates was measured and enzyme kinetics constants kcat, KM, kcat/KM and ki were determined. Metal oxide particles presented an inhibitory effect similar to that of a competitive inhibitor, noticed through the increase value of the K M constant. Furthermore, PSPI conjugates had the highest inhibition of trypsin, illustrated by the significantly higher value of KM relative to the value for particles only.

Place, publisher, year, edition, pages
2019. , p. 14
Keywords [en]
Proteases inhibitors, bioconjugates, enzyme kinetics, inhibition
National Category
Biocatalysis and Enzyme Technology
Identifiers
URN: urn:nbn:se:uu:diva-397114OAI: oai:DiVA.org:uu-397114DiVA, id: diva2:1370546
Educational program
Master Programme in Applied Biotechnology
Presentation
2019-10-14, A2:122a, Husargatan 3 752 37, Uppsala, 11:00 (English)
Supervisors
Examiners
Available from: 2019-11-19 Created: 2019-11-15 Last updated: 2019-11-19Bibliographically approved

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Biology Education CentreDepartment of Chemistry - BMC
Biocatalysis and Enzyme Technology

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CiteExportLink to record
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Citation style
  • apa
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Output format
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