Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Yeast Volatomes Differentially Affect Larval Feeding in an Insect Herbivore
Mid Sweden University, Faculty of Science, Technology and Media, Department of Chemical Engineering.
Swedish Univ Agr Sci, Alnarp; Corp Colombiana Invest Agr AGROSAVIA, Mosquera, Colombia.
Swedish Univ Agr Sci, Alnarp; Czech Univ Life Sci, Prague, Czech Republic.
Swedish Univ Agr Sci, Alnarp/Ultuna.
Show others and affiliations
2019 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 85, no 21, article id e01761-19Article in journal (Refereed) Published
Abstract [en]

Yeasts form mutualistic interactions with insects. Hallmarks of this interaction include provision of essential nutrients, while insects facilitate yeast dispersal and growth on plant substrates. A phylogenetically ancient chemical dialogue coordinates this interaction, where the vocabulary, the volatile chemicals that mediate the insect response, remains largely unknown. Here, we used gas chromatography-mass spectrometry, followed by hierarchical cluster and orthogonal partial least-squares discriminant analyses, to profile the volatomes of six Metschnikowia spp., Cryptococcus nemorosus, and brewer's yeast (Saccharomyces cerevisiae). The yeasts, which are all found in association with insects feeding on foliage or fruit, emit characteristic, species-specific volatile blends that reflect the phylogenetic context. Species specificity of these volatome profiles aligned with differential feeding of cotton leafworm (Spodoprera littoralis) larvae on these yeasts. Bioactivity correlates with yeast ecology; phylloplane species elicited a stronger response than fruit yeasts, and larval discrimination may provide a mechanism for establishment of insect-yeast associations. The yeast volatomes contained a suite of insect attractants known from plant and especially floral headspace, including (Z)-hexenyl acetate, ethyl (2E,4Z)-deca-2,4-dienoate (pear ester), (3E)-4,8-dimethylnona-1,3,7-triene (DMNT), linalool, alpha-terpineol, beta-myrcene, or (E,E)-alpha-farnesene. A wide overlap of yeast and plant volatiles, notably floral scents, further emphasizes the prominent role of yeasts in plant-microbe-insect relationships, including pollination. The knowledge of insect-yeast interactions can be readily brought to practical application, as live yeasts or yeast metabolites mediating insect attraction provide an ample tool-box for the development of sustainable insect management. IMPORTANCE Yeasts interface insect herbivores with their food plants. Communication depends on volatile metabolites, and decoding this chemical dialogue is key to understanding the ecology of insect-yeast interactions. This study explores the volatomes of eight yeast species which have been isolated from foliage, from flowers or fruit, and from plant-feeding insects. These yeasts each release a rich bouquet of volatile metabolites, including a suite of known insect attractants from plant and floral scent. This overlap underlines the phylogenetic dimension of insect-yeast associations, which according to the fossil record long predate the appearance of flowering plants. Volatome composition is characteristic for each species, aligns with yeast taxonomy, and is further reflected by a differential behavioral response of cotton leafworm larvae, which naturally feed on foliage of a wide spectrum of broad-leaved plants. Larval discrimination may establish and maintain associations with yeasts and is also a substrate for designing sustainable insect management techniques.

Place, publisher, year, edition, pages
2019. Vol. 85, no 21, article id e01761-19
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:miun:diva-37671DOI: 10.1128/AEM.01761-19ISI: 000490946700023PubMedID: 31444202Scopus ID: 2-s2.0-85073483905OAI: oai:DiVA.org:miun-37671DiVA, id: diva2:1369974
Available from: 2019-11-13 Created: 2019-11-13 Last updated: 2020-01-23Bibliographically approved
In thesis
1. Some Approaches to Eco-Friendly Products from Natural Matrices
Open this publication in new window or tab >>Some Approaches to Eco-Friendly Products from Natural Matrices
2020 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Since the onset of the industrial and chemical revolution, humans have caused immense damages to the surrounding flora and fauna. Effective methods for wood protection measures proved to be toxic; fossil fuels contribute to global warming and pesticides can be detected in the air, water, and soil. It is abundantly clear that efforts to find eco-friendly products are needed, while simultaneously providing the necessary incentives for sustainable worldwide development. Using renewable resources play a critical role in this shift towards circular economies.

Wood has long been used as a renewable resource in high demand, but its susceptibility to attack by wood-decaying fungi mean that most European woods need to be protected against these fungi before outdoor use. We showed that fractionating turpentine, a pulp and paper mill by-product, increased antifungal efficacy by concentrating bioactive oxygenated sesquiterpenes. Based on this result, recombinations of the fractions were shown to exhibit synergistic effects that enable a more efficient product utilisation. In addition, this approach enabled putative identifications of previously unknown Picea abies turpentine constituents present at low levels.

For a carbon-neutral society, production of biofuels using oleaginous yeast to convert lignocellulosic biomass into fuel has been hailed as a next-generation source of bioenergy. However, lignocellulose biofuel production by microorganisms is not straightforward and one challenge is the formation of microbe-toxic monomers, such as vanillin, during lignin degradation. The oleaginous yeast Cystobasidium laryngis and other potential oil-producing yeasts were screened for their viability and vanillin biotransformation capabilities. To this end, a mass chromatographic peak extraction tool termed TMATE was developed. Vanillyl alcohol was found to be the main product following vanillin degradation.

The detrimental health and ecological effects of pesticides highlight the urgency for alternative crop protection measures, such as biological insect control and semiochemicals. In this regard, we present an essential step towards understanding the varied chemical ecology of microbe-insect interactions. Our methodology and findings provide cues with high information value that can be used to develop well-informed and potentially sustainable pest management regimes by, for example, the push-pull methodology using live yeasts.

Place, publisher, year, edition, pages
Sundsvall: Mid Sweden University, 2020. p. 96
Series
Mid Sweden University doctoral thesis, ISSN 1652-893X ; 312
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:miun:diva-38176 (URN)978-91-88947-13-0 (ISBN)
Public defence
2020-01-31, O102, Holmgatan 10, Sundsvall, 10:30 (English)
Opponent
Supervisors
Note

Vid tidpunkten för disputationen var följande delarbeten opublicerade: delarbete 1 (inskickat), delarbete 2 (accepterat), delarbete 4 (manuskript).

At the time of the doctoral defence the following papers were unpublished: paper 1 (submitted), paper 2 (accepted), paper 4 (manuscript).

Available from: 2020-01-08 Created: 2020-01-03 Last updated: 2020-01-08Bibliographically approved

Open Access in DiVA

fulltext(1053 kB)30 downloads
File information
File name FULLTEXT01.pdfFile size 1053 kBChecksum SHA-512
41eb131c8e3438a9d11fb8a94d5a872cd3218bf08f6f6bbe7ecf7fbf946ad0beeb29d791e20d2f66b80456ad570c1645d1af2c81fb443e068642d2c2c6265441
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Ljunggren, JoelHedenström, ErikWitzgall, Peter
By organisation
Department of Chemical Engineering
In the same journal
Applied and Environmental Microbiology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 30 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 46 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf