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Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in Mammals
Agr Res Org, Volcani Ctr, Dept Anim Sci, POB 15159, IL-7528809 Rishon Letsiyon, Israel.
Charles Univ Prague, Fac Sci, Dept Dev Biol, Prague 12843, Czech Republic;McMaster Univ, Fac Sci, Dept Biol, Hamilton, ON L8S 4K1, Canada.
Univ Toulouse, GenPhySE, INRA, ENVT, F-31326 Castanet Tolosan, France.ORCID iD: 0000-0002-1477-7633
Czech Acad Sci, Inst Mol Genet, Prague 14220, Czech Republic.
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2019 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, no 18, article id 4489Article in journal (Refereed) Published
Abstract [en]

In mammals, leptin and tumor-necrosis factor (TNF) are prominent interacting adipokines mediating appetite control and insulin sensitivity. While TNF pleiotropically functions in immune defense and cell survival, leptin is largely confined to signaling energy stores in adipocytes. Knowledge about the function of avian leptin and TNF is limited and they are absent or lowly expressed in adipose, respectively. Employing radiation-hybrid mapping and FISH-TSA, we mapped TNF and its syntenic genes to chicken chromosome 16 within the major histocompatibility complex (MHC) region. This mapping position suggests that avian TNF has a role in regulating immune response. To test its possible interaction with leptin within the immune system and beyond, we compared the transcription patterns of TNF, leptin and their cognate receptors obtained by meta-analysis of GenBank RNA-seq data. While expression of leptin and its receptor (LEPR) were detected in the brain and digestive tract, TNF and its receptor mRNAs were primarily found in viral-infected and LPS-treated leukocytes. We confirmed leptin expression in the duodenum by immunohistochemistry staining. Altogether, we suggest that whereas leptin and TNF interact as adipokines in mammals, in birds, they have distinct roles. Thus, the interaction between leptin and TNF may be unique to mammals.

Place, publisher, year, edition, pages
2019. Vol. 20, no 18, article id 4489
Keywords [en]
radiation-hybrid mapping, FISH-TSA, chicken, TNF, immune system, leptin, digestive tract, duodenum
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-396670DOI: 10.3390/ijms20184489ISI: 000489100500168PubMedID: 31514326OAI: oai:DiVA.org:uu-396670DiVA, id: diva2:1368596
Available from: 2019-11-07 Created: 2019-11-07 Last updated: 2019-11-07Bibliographically approved

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