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The unique photosynthetic apparatus of Pinaceae: analysis of photosynthetic complexes in Picea abies
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2019 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 70, no 12, p. 3211-3225Article in journal (Refereed) Published
Abstract [en]

Pinaceae are the predominant photosynthetic species in boreal forests, but so far no detailed description of the protein components of the photosynthetic apparatus of these gymnosperms has been available. In this study we report a detailed characterization of the thylakoid photosynthetic machinery of Norway spruce (Picea abies (L.) Karst). We first customized a spruce thylakoid protein database from translated transcript sequences combined with existing protein sequences derived from gene models, which enabled reliable tandem mass spectrometry identification of P. abies thylakoid proteins from two-dimensional large pore blue-native/SDS-PAGE. This allowed a direct comparison of the two-dimensional protein map of thylakoid protein complexes from P. abies with the model angiosperm Arabidopsis thaliana. Although the subunit composition of P. abies core PSI and PSII complexes is largely similar to that of Arabidopsis, there was a high abundance of a smaller PSI subcomplex, closely resembling the assembly intermediate PSI* complex. In addition, the evolutionary distribution of light-harvesting complex (LHC) family members of Pinaceae was compared in silico with other land plants, revealing that P. abies and other Pinaceae (also Gnetaceae and Welwitschiaceae) have lost LHCB4, but retained LHCB8 (formerly called LHCB4.3). The findings reported here show the composition of the photosynthetic apparatus of P. abies and other Pinaceae members to be unique among land plants.

Place, publisher, year, edition, pages
Oxford University Press, 2019. Vol. 70, no 12, p. 3211-3225
Keywords [en]
Blue native gel, conifer, light harvesting, photosystem, Picea abies (Norway spruce), Pinaceae, thylakoid protein complexes
National Category
Botany Biochemistry and Molecular Biology
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URN: urn:nbn:se:umu:diva-164434DOI: 10.1093/jxb/erz127ISI: 000484414200017PubMedID: 30938447OAI: oai:DiVA.org:umu-164434DiVA, id: diva2:1362968
Available from: 2019-10-22 Created: 2019-10-22 Last updated: 2019-10-22Bibliographically approved

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Grebe, SteffenTrotta, AndreaGollan, Peter J.Jansson, StefanTikkanen, Mikko
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Umeå Plant Science Centre (UPSC)Department of Plant Physiology
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