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Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
All India Inst Med Sci, Dept Biophys, New Delhi 110029, India.ORCID iD: 0000-0003-4471-9546
All India Inst Med Sci, Dept Biophys, New Delhi 110029, India.
All India Inst Med Sci, CDER, Dept Oral & Maxillofacial Surg, New Delhi 110029, India.
All India Inst Med Sci, Dept Biophys, New Delhi 110029, India.
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2019 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, no 16, article id 4035Article in journal (Refereed) Published
Abstract [en]

The importance of whole protein extracts from different types of human teeth in modulating the process of teeth biomineralization is reported. There are two crucial features in protein molecules that result in efficient teeth biomineralization. Firstly, the unique secondary structure characteristics within these proteins i.e. the exclusive presence of a large amount of intrinsic disorder and secondly, the presence of post-translational modifications (PTM) like phosphorylation and glycosylation within these protein molecules. The present study accesses the structural implications of PTMs in the tooth proteins through scanning electron microscopy and transmission electron microscopy. The deglycosylated/dephosphorylated protein extracts failed to form higher-order mineralization assemblies. Furthermore, through nanoparticle tracking analysis (NTA) we have shown that dephosphorylation and deglycosylation significantly impact the biomineralization abilities of the protein extract and resulted in smaller sized clusters. Hence, we propose these post-translational modifications are indispensable for the process of teeth biomineralization. In addition to basic science, this study would be worth consideration while designing of biomimetics architecture for an efficient peptide-based teeth remineralization strategy.

Place, publisher, year, edition, pages
MDPI, 2019. Vol. 20, no 16, article id 4035
Keywords [en]
human teeth, biomineralization, intrinsically disordered proteins, post-translational modifications, scanning electron microscopy, transmission electron microscopy
National Category
Dentistry
Identifiers
URN: urn:nbn:se:uu:diva-394640DOI: 10.3390/ijms20164035ISI: 000484411100192PubMedID: 31430851OAI: oai:DiVA.org:uu-394640DiVA, id: diva2:1362045
Available from: 2019-10-17 Created: 2019-10-17 Last updated: 2019-10-17Bibliographically approved

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