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Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches
Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Cheongju 28644, Chungbuk, South Korea.
Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Cheongju 28644, Chungbuk, South Korea;GIST, Sch Life Sci, Gwangju 61005, South Korea.
Uppsala University, Disciplinary Domain of Science and Technology, Earth Sciences, Department of Earth Sciences, Palaeobiology. Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Cheongju 28644, Chungbuk, South Korea.
Chung Ang Univ, Dept Life Sci, Seoul 156756, South Korea.
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2019 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, no 16, article id 3994Article in journal (Refereed) Published
Abstract [en]

Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway.

Place, publisher, year, edition, pages
MDPI, 2019. Vol. 20, no 16, article id 3994
Keywords [en]
Antistasin, Helobdella, in situ hybridization, expression, hedgehog signaling
National Category
Genetics
Identifiers
URN: urn:nbn:se:uu:diva-394639DOI: 10.3390/ijms20163994ISI: 000484411100151PubMedID: 31426335OAI: oai:DiVA.org:uu-394639DiVA, id: diva2:1362043
Available from: 2019-10-17 Created: 2019-10-17 Last updated: 2019-10-17Bibliographically approved

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