Titanium alloys find wide application in the medical implants industry, which includes areas of orthopaedic and dental implants. The reason for the popularity of the material is high mechanical strength, low density, and reported growth of bone onto the material, as well as corrosion resistance. Despite the general success of titanium materials, a drawback is that it is vulnerable to bacterial colonization, which can cause implant failure through inflammatory diseases. Peri-implantitis is one such disease, which can lead to irreversible bone loss and subsequently implant instability.

This thesis focuses on the use of copper (Cu) as an antibacterial element in titanium alloys, where the purpose is designing inherently antibacterial materials.

With an understanding that copper can reduce bacterial populations by ion release of Cu into solutions, as well as by direct contact of bacteria with Cu surfaces: studies on the effect of Cu ions on bacteria and cells were conducted, in addition to studies on Ti-Cu_x alloys.

Varying Cu concentrations in solution were introduced to bacteria (Staphylococcus epidermidis) and cells (MC3T3 murine calvarial osteoblasts), and it was found that the lethal dosage for Cu ions was in the range from 9x10^-5 to 9x10^-6 g/ml, for bacteria and cells. The Cu ions were also found to cause a stress response for this bacteria at concentrations between 9x10^-6 to 9x10^-7 g/ml, and recommended to be avoided for implant materials.

For Ti-Cu_x binary alloys, studies established that a 10wt%Cu alloy, which released 9x10^-8 g/ml, reduced the bacterial population by 27 % in 6 hours in a direct contact test. This alloy was found to be composed of intermetallic (Ti₂Cu) and hexagonal closed packed titanium (HCP-Ti) crystals. A separate study on aged heat treated Ti-Cu_x alloys, showed that an additional phase of Ti₃Cu was present in lower volume fraction. The aged alloys of Ti-Cu_x showed higher volume fraction of Ti₂Cu but only a slightly higher antibacterial ability, compared to those without ageing. The hardness of the Ti-Cu_x alloys was however detrimentally affected by ageing, especially for the 10wt%Cu alloy.

Investigations on the alloying of Cu with an existing implant alloy, Ti-10wt%Ta-1.6wt%Nb-1.7wt%Zr (TNTZ), was also performed and at higher wt%Cu alloys with three-phased microstructures were present. Alloying of Cu in the TNTZ material increased hardness and with further development of this novel alloy, a potential biomaterial for clinical applications could be designed.

In conclusion, the results of this thesis demonstrate that the use of Cu in proximity to cells and bacteria requires dose dependent consideration for material design, so that antibacterial materials can be developed that do not harm tissue. The appropriate design of alloys can also be performed so as to allow antibacterial ability to be achieved, along with ensuring appropriate mechanical and corrosion properties. Furthermore, Cu as an antibacterial element can be alloyed into various titanium alloy systems and with further development in this area; antibacterial alloys could benefit the implant industry and patients alike.