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Development of a method for kinetic characterisation of therapeutic antibodies in solution using the Gyrolab platform
2019 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Therapeutic antibodies dominate the pharmaceutical market and improve the lives of millions of people annually. One important step when developing new medicines is to kinetically characterise the drug candidates. For antibodies this is difficult since many antibody reactions are extremely slow. By combining a mathematical formula that was recently published with the well-established technology from Gyros Protein Technologies, a new method for full kinetic characterization was developed and tested in this master thesis. The method provided precise data for five antibodies while also proving to be highly reproducible. By using small sample volumes, unlabelled reagents and having the reaction proceed in solution, this method offers advantages compared to many conventional approaches. 

Place, publisher, year, edition, pages
2019. , p. 33
Series
UPTEC X ; 19026
Keywords [en]
kinetics, affinity, gyros, antibodies, antibody, kinetic, association, dissociation, affinity chromatography
National Category
Biophysics
Identifiers
URN: urn:nbn:se:uu:diva-393505OAI: oai:DiVA.org:uu-393505DiVA, id: diva2:1358364
External cooperation
Gyros Protein Technologies
Educational program
Molecular Biotechnology Engineering Programme
Presentation
2019-05-31, Husargatan 3, Uppsala, 19:52 (Swedish)
Supervisors
Examiners
Available from: 2019-10-08 Created: 2019-10-07 Last updated: 2019-10-08Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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