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Real-time opto-tracing of curli and cellulose in live Salmonella biofilms using conjugated oligothiophenes
Karolinska Institutet, Stockholm, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
Karolinska Institutet, Stockholm, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
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2016 (English)In: npj Biofilms and Microbiomes, ISSN 2055-5008, Vol. 2, article id 16024Article in journal (Refereed) Published
Abstract [en]

Extracellular matrix (ECM) is the protein- and polysaccharide-rich backbone of bacterial biofilms that provides a defensive barrier in clinical, environmental and industrial settings. Understanding the dynamics of biofilm formation in native environments has been hindered by a lack of research tools. Here we report a method for simultaneous, real-time, in situ detection and differentiation of the Salmonella ECM components curli and cellulose, using non-toxic, luminescent conjugated oligothiophenes (LCOs). These flexible conjugated polymers emit a conformation-dependent fluorescence spectrum, which we use to kinetically define extracellular appearance of curli fibres and cellulose polysaccharides during bacterial growth. The scope of this technique is demonstrated by defining biofilm morphotypes of Salmonella enterica serovars Enteritidis and Typhimurium, and their isogenic mutants in liquid culture and on solid media, and by visualising the ECM components in native biofilms. Our reported use of LCOs across a number of platforms, including intracellular cellulose production in eukaryotic cells and in infected tissues, demonstrates the versatility of this optotracing technology, and its ability to redefine biofilm research.

Place, publisher, year, edition, pages
Nature Publishing Group, 2016. Vol. 2, article id 16024
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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
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URN: urn:nbn:se:liu:diva-160729DOI: 10.1038/npjbiofilms.2016.24ISI: 000419466300019PubMedID: 28721253Scopus ID: 2-s2.0-85020828677OAI: oai:DiVA.org:liu-160729DiVA, id: diva2:1357871
Available from: 2019-10-04 Created: 2019-10-04 Last updated: 2019-12-03Bibliographically approved

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Bäck, MarcusJohansson, Leif B. G.Nilsson, Peter
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