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Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. Karolinska Inst, Dept Clin Neurosci, St Erik Eye Hosp, Stockholm, Sweden.ORCID iD: 0000-0002-9938-2808
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.ORCID iD: 0000-0002-4597-041x
2019 (English)In: British Journal of Ophthalmology, ISSN 0007-1161, E-ISSN 1468-2079, Vol. 103, no 8, p. 1190-1194Article in journal (Refereed) Published
Abstract [en]

Background/aims Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased concentration of beta-crystalline B2 (CRYBB2) was observed in the aqueous humour (AH) in eyes with PEX in a pooled material. Here, the protein content was examined on individual basis. Methods During cataract surgery, AH was sampled from patients with and without PEX, 10 eyes in each group. The proteins were digested and labelled with isotopomeric dimethyl labels, separated with high-pressure liquid chromatography and analysed in an Orbitrap mass analyzer. Results The concentration of complement factor 3, kininogen-1, antithrombin III and vitamin D-binding protein was increased in all eyes with PEX. Retinol-binding protein 3, glutathione peroxidase, calsyntenin-1 and carboxypeptidase E were decreased in eyes with PEX. Beta-crystalline B1 and CRYBB2 and gamma-crystalline D were up to eightfold upregulated in 4 of 10 in eyes with PEX. Conclusion The results indicate that oxidative stress and inflammation are contributing factors in the formation of PEX. Knowledge about the proteome in PEX is relevant for understanding this condition.

Place, publisher, year, edition, pages
BMJ PUBLISHING GROUP , 2019. Vol. 103, no 8, p. 1190-1194
Keywords [en]
aqueous humour, diagnostic tests, Investigation
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:uu:diva-393343DOI: 10.1136/bjophthalmol-2017-310416ISI: 000478915300031PubMedID: 30842085OAI: oai:DiVA.org:uu-393343DiVA, id: diva2:1355235
Funder
Swedish Research Council, 621-2011-4423Swedish Research Council, 2015-4870Available from: 2019-09-27 Created: 2019-09-27 Last updated: 2019-09-27Bibliographically approved

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Botling Taube, AmelieKonzer, AnneAlm, AlbertBergquist, Jonas
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