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Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice
Linköping University, Department of Clinical and Experimental Medicine, Divison of Neurobiology. Linköping University, Faculty of Medicine and Health Sciences. German Ctr Neurodegenerat Dis, Germany.
Univ Med Ctr Hamburg Eppendorf, Germany.
Univ Med Ctr Hamburg Eppendorf, Germany.
Univ Med Ctr Hamburg Eppendorf, Germany.
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2019 (English)In: PLoS biology, ISSN 1544-9173, E-ISSN 1545-7885, Vol. 17, no 8, article id e3000374Article in journal (Refereed) Published
Abstract [en]

A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE , 2019. Vol. 17, no 8, article id e3000374
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Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-160436DOI: 10.1371/journal.pbio.3000374ISI: 000483408500015PubMedID: 31393866OAI: oai:DiVA.org:liu-160436DiVA, id: diva2:1353451
Note

Funding Agencies|Knut and Alice Wallenberg Foundation; German Center for Neurodegenerative Diseases; German Research Foundation [SFB 1089]; ERA-NET NEURON (MicroSynDep, MicroSchiz); DFG; BMBF (IDSN) grant [BO4221, SFB 1286 Z2]; Helmholtz iMed grant; VW German-Israeli grant

Available from: 2019-09-23 Created: 2019-09-23 Last updated: 2019-11-28

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