In this work, the influence of different cooling rates (5, 20, 50 and 100 °C/s) on the microstructure and mechanical properties of Laser Metal Wire Deposited (LMwD) Ti-6Al-4V was investigated, this was done using a thermal-mechanical physical simulation system (Gleeble 3800, DSI). Two different soak times above β transus (held at 1100 °C), 5 and 40 s, were used and after cooling to 150 °C, the samples were tensile tested. The samples were characterized with optical microscopy (OM) and scanning electron microscopy (SEM) and hardness testing. The results were then compared, both with each other and with two reference samples, that were only heated to 150 °C and then tensile tested. It was found that for the lowest cooling rate, 5 °C/s, the microstructure had transformed from a basketweave α microstructure to a colony α microstructure in the center of the specimen waist where heating was most efficient. Ultimate tensile strength (UTS) was found to be in the range of 858 – 977 MPa, with the highest average being recorded for the reference samples, similar results were noted for the strain, with a range of  ⁓5 – 14 %, where the highest recorded average was for the reference samples. However, the extensometer used was not optimized for this kind of test, therefore percent reduction of area (RA) measurements were performed. The RA measurements produced a significantly different result than that obtained from the testing, a large scatter in the ductility was found, possibly due to thermal instability that occurred during testing. Overall, the microstructure appears to be relatively stable over the cooling range of 20 - 100 °C/s, no major differences were observed, the microstructure consisted of a homogeneous basketweave α microstructure, with little to no change in the measured average α lath thickness.