Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A continuous on-chip droplet washing platform with high bead recovery by acoustofluidics
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0001-7960-5878
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0001-7980-376x
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0002-1264-1337
2019 (English)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

Acoustofluidics is a promising technology for manipulation of fluids and particles in microchannels, and the technology has the ability to sort beads and cells in continuous flow with very high efficiency. Recently acoustofluidics has also been applied in segmental flow for positioning beads inside droplets. Compared with single-phase systems, droplet microfluidics has the advantages of faster reactions, lower cross-contamination and higher throughput. Moreover, the small size of the droplets makes them ideal as cultivation and reaction vials for single cell analysis. However, as the droplets are so small one challenge is to wash the droplets before image analysis. P. Mary et al. developed a microfluidic platform for droplet wash, whichis based on electrocoalescence and droplet break-ups with equal volume. The background noise was decreased significantly, however the recovery of the encapsulated cells was low. Alternative solutions have been presented by H. Lee et al. and S.R. Doonan et al. but as the bead recovery is controlled via magnetophoresis, the technology is only applicable to magnetic samples. Here we present a droplet microfluidic platform that enables background dilution with high bead recovery in a label-free manner using acoustophoresis.

Place, publisher, year, edition, pages
2019.
Keywords [en]
Droplet dilution, acoustophoresis, pico-injection, microfluidics, electrocoalescence
National Category
Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-392166OAI: oai:DiVA.org:uu-392166DiVA, id: diva2:1347096
Conference
Acoustofluidics 2019, 26-28 August 2019, Enschede, Netherlands
Available from: 2019-08-30 Created: 2019-08-30 Last updated: 2019-08-30Bibliographically approved

Open Access in DiVA

fulltext(275 kB)31 downloads
File information
File name FULLTEXT01.pdfFile size 275 kBChecksum SHA-512
2702ffcff447936dcb056a0b9313e2c43eb124c7c0a4e143e22856b2ebd7dbc7b5881a7610d5f58f04021b7643073b26f2b2025f0105e7dd78322b6659d538ab
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Liu, ZhenhuaFornell, AnnaTenje, Maria
By organisation
Microsystems TechnologyScience for Life Laboratory, SciLifeLab
Medical Laboratory and Measurements Technologies

Search outside of DiVA

GoogleGoogle Scholar
Total: 31 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 105 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf