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Possible Involvement of Intracellular Calcium-Independent Phospholipase A2 in the Release of Secretory Phospholipases from Mast Cells: Increased Expression in Ileal Mast Cells of Crohns Disease
Linnaeus Univ, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-6820-0215
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Surgery in Linköping.ORCID iD: 0000-0002-3250-5367
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2019 (English)In: CELLS, ISSN 2073-4409, Vol. 8, no 7, article id 672Article in journal (Refereed) Published
Abstract [en]

Increased activity of secretory phospholipases A(2) (sPLA(2)) type-II was previously observed in ileum of Crohns disease (CD). Our aims were to explore the involvement of calcium-independent (i)PLA(2 beta) in the release of sPLA(2)s from the human mast cell (MC) line (HMC-1) and investigate expressions of cytosolic (c)PLA(2) alpha, iPLA(2)beta, sPLA(2)-IIA and sPLA(2)-V in MCs of CD ileum. The release of sPLA(2) was investigated in HMC-1 by immunocytochemistry and ELISA. The expression intensities of PLA(2)s in mucosal MCs, and the proportion of PLA(2)-positive MCs, were investigated in normal ileum and in ileum from patients with CD by immunohistochemistry. The calcium ionophore-stimulated release of sPLA(2)-IIA and sPLA(2)-V from HMC-1 was reduced by the iPLA(2)-inhibitor bromoenol lactone. All four PLA(2)s were detectable in mucosal MCs, both in normal ileum and in CD, but the proportion of iPLA(2)beta-containing mucosal MCs and the expression intensity of sPLA(2)-IIA was increased in CD. Results indicate that iPLA(2)beta is involved in the secretion of sPLA(2)s from HMC-1, and suggest that iPLA(2)beta-mediated release of sPLA(2) from intestinal MCs may contribute to CD pathophysiology. Ex vivo studies on isolated mucosal mast cells are however needed to clarify the precise role of MC PLA(2)s in the inflammatory processes of CD.

Place, publisher, year, edition, pages
MDPI, 2019. Vol. 8, no 7, article id 672
Keywords [en]
phospholipases A(2); mast cells; Crohns disease; inflammation
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-159895DOI: 10.3390/cells8070672ISI: 000478902000073PubMedID: 31277247OAI: oai:DiVA.org:liu-159895DiVA, id: diva2:1346138
Note

Funding Agencies|Medical Research Council of Southeast Sweden; Faculty of Health and Life Sciences, Linneaus University, Sweden; Swedish Research Council VR-Medicine and Health [2014-02537, 2017-02475]; LIONS international Foundation

Available from: 2019-08-27 Created: 2019-08-27 Last updated: 2019-11-20Bibliographically approved

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Keita, ÅsaTinnerfelt Winberg, MartinSöderholm, Johan D
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