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Patient-derived organoid culture for 3D culture of colorectal cancer, renal cancer and osteosarcoma
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
2019 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

It is always important to choose appropriate anticancer drugs for cancer patients. At RCL, a division of Uppsala university hospital, drug resistance profiles of patients are evaluated by a cell viability assay called FMCA. However, the number of anticancer drugs that can be evaluated by the FMCA is dependent on the number of viable cancer cells from tissues that can be obtained from each individual patient. Therefore, improvement of cell viability methods is an important issue at RCL. This study was performed to improve the FMCA method by organoid culture from colorectal cancer, renal cancer and osteosarcoma to increase the number of cancer cells. As results, it was successful to expand cryopreserved patient cancer cells to organoids to acquire more cells than before expansion. Organoids showed rounded structure in microscopy images. Thereafter, FMCA was performed on organoids as well as on thawed cryopreserved cancer cells from the original sample. Those results showed that original cancer cells, cryopreserved original cancer cells and expanded organoids derived from those cryopreserved cells had similar resistance profiles. It was also discovered that the organoids secreted VEGF under the cultivation. From those results, it can be concluded that organoids are representative of the original cancer from the patients. It is however needed to improve organoid culture methods, and to further confirm organoids by protein expression analysis and DNA analysis.

Place, publisher, year, edition, pages
2019. , p. 29
Keywords [en]
FMCA, Anticancer drugs, Survival index, BME-gel, VEGF
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-391043OAI: oai:DiVA.org:uu-391043DiVA, id: diva2:1344438
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
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Available from: 2019-08-21 Created: 2019-08-20 Last updated: 2019-08-21Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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