Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas
Univ Rennes, CNRS, IGDR, UMR 6290, F-35000 Rennes, France.ORCID iD: 0000-0003-1714-437X
Univ Rennes, CNRS, IGDR, UMR 6290, F-35000 Rennes, France.
Univ Rennes, CNRS, IGDR, UMR 6290, F-35000 Rennes, France.
Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Dr Aiguader 88, Barcelona 08003, Spain.ORCID iD: 0000-0002-1331-4549
Show others and affiliations
2019 (English)In: Genes, ISSN 2073-4425, E-ISSN 2073-4425, Vol. 10, no 6, article id 477Article in journal (Refereed) Published
Abstract [en]

Mucosal melanomas (MM) are rare aggressive cancers in humans, and one of the most common forms of oral cancers in dogs. Similar biological and histological features are shared between MM in both species, making dogs a powerful model for comparative oncology studies of melanomas. Although exome sequencing recently identified recurrent coding mutations in canine MM, little is known about changes in non-coding gene expression, and more particularly, in canine long non-coding RNAs (lncRNAs), which are commonly dysregulated in human cancers. Here, we sampled a large cohort (n = 52) of canine normal/tumor oral MM from three predisposed breeds (poodles, Labrador retrievers, and golden retrievers), and used deep transcriptome sequencing to identify more than 400 differentially expressed (DE) lncRNAs. We further prioritized candidate lncRNAs by comparative genomic analysis to pinpoint 26 dog-human conserved DE lncRNAs, including SOX21-AS, ZEB2-AS, and CASC15 lncRNAs. Using unsupervised co-expression network analysis with coding genes, we inferred the potential functions of the DE lncRNAs, suggesting associations with cancer-related genes, cell cycle, and carbohydrate metabolism Gene Ontology (GO) terms. Finally, we exploited our multi-breed design to identify DE lncRNAs within breeds. This study provides a unique transcriptomic resource for studying oral melanoma in dogs, and highlights lncRNAs that may potentially be diagnostic or therapeutic targets for human and veterinary medicine.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 10, no 6, article id 477
Keywords [en]
mucosal melanoma, dogs, transcriptome sequencing, long non-coding RNAs (lncRNAs)
National Category
Genetics
Identifiers
URN: urn:nbn:se:uu:diva-390645DOI: 10.3390/genes10060477ISI: 000473797000072PubMedID: 31234577OAI: oai:DiVA.org:uu-390645DiVA, id: diva2:1343787
Available from: 2019-08-19 Created: 2019-08-19 Last updated: 2019-08-19Bibliographically approved

Open Access in DiVA

fulltext(2616 kB)43 downloads
File information
File name FULLTEXT01.pdfFile size 2616 kBChecksum SHA-512
d5986f8b1a7d02d899479daf6f1be7b19995e2faed4660a2cd4fb847406018e29ae3b15b548d8c49cc146397dfac06a9c26b715bad419c882df0751fcf622a24
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hitte, ChristopheWucher, ValentinHedan, BenoitLindblad-Toh, KerstinDerrien, Thomas
By organisation
Department of Medical Biochemistry and MicrobiologyScience for Life Laboratory, SciLifeLab
In the same journal
Genes
Genetics

Search outside of DiVA

GoogleGoogle Scholar
Total: 43 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 49 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf