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Characterization of protein extracts from different types of human teeth and insight in biomineralization
AIIMS, Dept Biophys, New Delhi, India.ORCID iD: 0000-0003-4471-9546
Jamia Hamdard, Dept Biochem, New Delhi, India.
AIIMS, Dept Oral & Maxillofacial Surg, CDER, New Delhi, India.
AIIMS, Dept Biophys, New Delhi, India.
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2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 9314Article in journal (Refereed) Published
Abstract [en]

The present study describes an efficient method for isolation and purification of protein extracts from four types of human teeth i.e. molar, premolar, canine, and incisor. Detailed structural characterization of these protein extracts was done by Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD) which showed that a major fraction of the proteins present are unstructured in nature including primarily random coils in addition to the other structures like extended beta (beta) structure, poly-l-proline-type II (PPII) helix, turns, with only a small fraction constituting of ordered structures like alpha (alpha) helix and beta sheets. These resultant labile structures give the proteins the necessary flexibility that they require to interact with a variety of substrates including different ions like calcium and phosphates and for other protein-protein interactions. We also did initial studies on the mineralization of calcium phosphate with the protein extracts. Nanoparticle tracking analysis (NTA) show an increase in the size of calcium phosphate accumulation in the presence of protein extracts. We propose that protein extracts elevate the crystallization process of calcium phosphate. Our current biophysical study provides novel insights into the structural characterization of proteins from human teeth and their implications in understanding the tooth biomineralization. As per our knowledge, this is the first report which focuses on the whole protein extraction from different types of human teeth as these extracts imitate the in vivo tooth mineralization.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2019. Vol. 9, article id 9314
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Dentistry
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URN: urn:nbn:se:uu:diva-390817DOI: 10.1038/s41598-019-44268-2ISI: 000472999700006PubMedID: 31249316OAI: oai:DiVA.org:uu-390817DiVA, id: diva2:1343412
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VinnovaAvailable from: 2019-08-16 Created: 2019-08-16 Last updated: 2019-08-16Bibliographically approved

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