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Effects of Long-Term Storage at -80 degrees C on the Human Plasma Metabolome
Metan Hlth GmbH, Tegeler Weg 33, D-10589 Berlin, Germany.
Biocrates Life Sci AG, Eduard Bodem Gasse 8, A-6020 Innsbruck, Austria.
Metan Hlth GmbH, Tegeler Weg 33, D-10589 Berlin, Germany.
Metan Hlth GmbH, Tegeler Weg 33, D-10589 Berlin, Germany.
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2019 (English)In: Metabolites, ISSN 2218-1989, E-ISSN 2218-1989, Vol. 9, no 5, article id 99Article in journal (Refereed) Published
Abstract [en]

High-quality biological samples are required for the favorable outcome of research studies, and valid data sets are crucial for successful biomarker identification. Prolonged storage of biospecimens may have an artificial effect on compound levels. In order to investigate the potential effects of long-term storage on the metabolome, human ethylenediaminetetraacetic acid (EDTA) plasma samples stored for up to 16 years were analyzed by gas and liquid chromatography-tandem mass spectrometry-based metabolomics. Only 2% of 231 tested plasma metabolites were altered in the first seven years of storage. However, upon longer storage periods of up to 16 years and more time differences of few years significantly affected up to 26% of the investigated metabolites when analyzed within subject age groups. Ontology classes that were most affected included complex lipids, fatty acids, energy metabolism molecules, and amino acids. In conclusion, the human plasma metabolome is adequately stable to long-term storage at -80 degrees C for up to seven years but significant changes occur upon longer storage. However, other biospecimens may display different sensitivities to long-term storage. Therefore, in retrospective studies on EDTA plasma samples, analysis is best performed within the first seven years of storage.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 9, no 5, article id 99
Keywords [en]
biomarker, long-term stability, storage, plasma, metabolomics, mass spectrometry
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-390538DOI: 10.3390/metabo9050099ISI: 000472660600019PubMedID: 31108909OAI: oai:DiVA.org:uu-390538DiVA, id: diva2:1342117
Funder
EU, FP7, Seventh Framework Programme, GA 306031Available from: 2019-08-12 Created: 2019-08-12 Last updated: 2019-08-12Bibliographically approved

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