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Mapping of human papilloma virus, p16, and epstein-barr virus in non-malignant tonsillar disease
Östersunds Hosp, Div Otorhinolaryngol, Dept Clin Sci, Ostersund, Sweden.
Östersunds Hosp, Div Otorhinolaryngol, Dept Clin Sci, Östersund, Sweden;Jämtland Hadedalen Cty Council, Östersund, Sweden.
Ostersunds Hosp, Div Clin Virol, Dept Clin Microbiol, Ostersund, Sweden.
Ostersunds Hosp, Div Clin Virol, Dept Clin Microbiol, Ostersund, Sweden.
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2019 (English)In: LARYNGOSCOPE INVESTIGATIVE OTOLARYNGOLOGY, ISSN 2378-8038, Vol. 4, no 3, p. 285-291Article in journal (Refereed) Published
Abstract [en]

Objectives Due to their location in the entrance of the aero-digestive tract, tonsils are steadily exposed to viruses. Human papilloma virus (HPV) and Epstein-Barr virus (EBV) are two potentially oncogenic viruses that tonsils encounter. The incidence of HPV positive tonsillar cancer is on the rise and it is unknown when infection with HPV occurs. Aim To investigate if tonsils are infected with HPV and EBV, to study the co-expression of HPV and its surrogate marker p16, and to evaluate the number of EBV positive cells in benign tonsillar disease. Materials and Methods Tonsils from 40 patients in a university hospital were removed due to hypertrophy, chronic or recurrent infection. These were analyzed for presence of HPV, its surrogate marker p16, and EBV. HPV was studied using PapilloCheck (a PCR method), while p16 was identified in epithelial and lymphoid tissue with immunohistochemistry and EBV using EBER-ISH (Epstein-Barr encoding region-in situ hybridization). Results HPV was not detected, and p16 was present at low numbers in all epithelial samples as well as in 92.5% of the lymphoid tonsillar samples. At least one EBER-positive cell was seen in 65% of cases. Larger numbers of EBER-expressing cells were only seen in two cases. Conclusion These findings demonstrate that EBV and HPV infect tonsils independently, but further studies are warranted to confirm their infectious relationship.

Place, publisher, year, edition, pages
WILEY , 2019. Vol. 4, no 3, p. 285-291
Keywords [en]
Human papillomavirus, Epstein-Barr virus, non-malignant tonsillar disease, EBER-ISH, PapilloCheck, immunohistochemistry
National Category
Microbiology in the medical area Otorhinolaryngology
Identifiers
URN: urn:nbn:se:uu:diva-390027DOI: 10.1002/lio2.260ISI: 000471907200002PubMedID: 31236460OAI: oai:DiVA.org:uu-390027DiVA, id: diva2:1341242
Funder
Västerbotten County CouncilAvailable from: 2019-08-08 Created: 2019-08-08 Last updated: 2019-08-08Bibliographically approved

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