Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Amino Acid-Dependent Alterations in Cell Wall and Cell Morphology of Deinococcus indicus DR1
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Show others and affiliations
2019 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 10, article id 1449Article in journal (Refereed) Published
Abstract [en]

Deinococcus radiodurans exhibits growth medium-dependent morphological variation in cell shape, but there is no evidence whether this phenomenon is observed in other members of the Deinococcaceae family. In this study, we isolated a red-pigmented, aerobic, Deinococcus indicus strain DR1 from Dadri wetland, India. This D. indicus strain exhibited cell-morphology transition from rod-shaped cells to multi-cell chains in a growth-medium-dependent fashion. In response to addition of 1% casamino acids in the minimal growth medium, rod-shaped cells formed multi-cell chains. Addition of all 20 amino acids to the minimal medium was able to recapitulate the phenotype. Specifically, a combination of L-methionine, L-lysine, L-aspartate, and L-threonine caused morphological alterations. The transition from rod shape to multi-cell chains is due to delay in daughter cell separation after cell division. Minimal medium supplemented with L-ornithine alone was able to cause cell morphology changes. Furthermore, a comparative UPLC analysis of PG fragments isolated from D. indicus cells propagated in different growth media revealed alterations in the PG composition. An increase in the overall cross-linkage of PG was observed in muropeptides from nutrient-rich TSB and NB media versus PYE medium. Overall our study highlights that environmental conditions influence PG composition and cell morphology in D. indicus.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019. Vol. 10, article id 1449
Keywords [en]
Deinococcus indicus, morphological alterations, amino acids, cell wall, muropeptides
National Category
Microbiology Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-161695DOI: 10.3389/fmicb.2019.01449ISI: 000473576800001OAI: oai:DiVA.org:umu-161695DiVA, id: diva2:1340498
Funder
Knut and Alice Wallenberg FoundationSwedish Research CouncilThe Kempe FoundationsAvailable from: 2019-08-05 Created: 2019-08-05 Last updated: 2019-08-05Bibliographically approved

Open Access in DiVA

fulltext(9477 kB)34 downloads
File information
File name FULLTEXT01.pdfFile size 9477 kBChecksum SHA-512
633747b0aede3e96bc1880c0856c42973549fc22e3ce6be1ac8d67637bfd1fa138dbf344a158041a2fb204cd561b945e28164c918fd248e4bc17a66fbf064fba
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Ritzl, BarbaraCava, Felipe
By organisation
Department of Molecular Biology (Faculty of Medicine)Molecular Infection Medicine Sweden (MIMS)Umeå Centre for Microbial Research (UCMR)
In the same journal
Frontiers in Microbiology
MicrobiologyMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 34 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 73 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf